Goat Anti-NR4A1 Polyclonal Antibody – Cat# MBS224277

Human thyroid cell lysate probed with Goat anti Human TR3 orphan receptor (MBS224277)

TR3 ORPHAN RECEPTOR (NR4A1) Antibody – Goat Polyclonal

Name: NR4A1 Antibody; Polyclonal; Goat; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS224277
Price: 710 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224277

MBS224277 specifically recognises human TR3 orphan receptor, otherwise known as NUR77, an immediate-early orphan nuclear receptor, and member of the nuclear receptor hormone family, encoded by the NR4A1 gene. TR3 orphan receptor plays a role in the mediation… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TR3 ORPHAN RECEPTOR (NR4A1), Polyclonal Antibody

Full Product Name

GOAT ANTI HUMAN TR3 ORPHAN RECEPTOR

Product Gene Name

anti-NR4A1 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Dog (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence)

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 0.5mg/ml (lot specific)

Specificity

This item specifically recognises human TR3 orphan receptor, otherwise known as NUR77, an immediate-early orphan nuclear receptor, and member of the nuclear receptor hormone family, encoded by the NR4A1 gene.
TR3 orphan receptor plays a role in the mediation of macrophage inflammatory responses, and in apoptosis and cell proliferation, through the binding to a variety of response elements. TR3 orphan receptor has been shown to bind to the anti-apoptotic protein Bcl-2, and can be induced by both physiological and physical stress-related stimuli, including tumour necrosis factor (TNF), inflammatory cytokines and magnetic fields.
This item is expected to recognise isoform a of TR3 orphan receptor.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

33,754 Da

OMIM

139139

3D Structure

ModBase 3D Structure for P22736

Perservative Stabilisers

0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin

Immunogen

Synthetic peptide sequence C-GRLPSKPKQPPDAS from the internal region of TR3 orphan receptor (NP_002126.2; NP_775180.1).
Buffer Solution||TRIS buffered saline

Antiserum Preparation

Antiserum to human TR3 orphan receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-NR4A1 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-NR4A1 antibody

Western Blot: This item detects a band of approximately 60kDa in human thyroid gland cell lysates.
ELISA: Minimum Dilution: 1/32000
Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 3.0ug/ml

Other Notes

Small volumes of anti-NR4A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NR4A1 purchase

MBS224277 is an antibody targeting NR4A1. Also known as GOAT ANTI HUMAN TR3 ORPHAN RECEPTOR. Reactive in Dog (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for NR4A1. It may not necessarily be applicable to this product.

NCBI GI #

320202956

NCBI GeneID

3164

NCBI Accession #

NP_001189162.1

UniProt Primary Accession #

P22736

UniProt Secondary Accession #

Q15627; Q53Y00; Q6IBU8; B4DML7

UniProt Related Accession #

P22736

GenBank Nucleotide #

NM_001202233.1

NCBI Official Full Name

nuclear receptor subfamily 4 group A member 1 isoform 2

NCBI Official Synonym Full Names

nuclear receptor subfamily 4, group A, member 1

NCBI Official Symbol

NR4A1

NCBI Official Synonym Symbols

HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77

UniProt Protein Name

Nuclear receptor subfamily 4 group A member 1

UniProt Synonym Protein Names

Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3

Protein Family

Nuclear receptor subfamily

UniProt Gene Name

NR4A1

UniProt Synonym Gene Names

GFRP1; HMR; NAK1; Nur77

UniProt Entry Name

NR4A1_HUMAN

NCBI Protein Information

nuclear receptor subfamily 4 group A member 1; ST-59; TR3 orphan receptor; early response protein NAK1; growth factor-inducible nuclear protein N10; hormone receptor; nerve growth factor IB nuclear receptor variant 1; nuclear hormone receptor NUR/77; orphan nuclear receptor HMR; orphan nuclear receptor TR3; steroid receptor TR3; testicular receptor 3

NCBI Summary for NR4A1

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

UniProt Comments for NR4A1

Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.

Protein type: DNA-binding; Nuclear receptor; Apoptosis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; signal transduction; cell migration during sprouting angiogenesis; innate immune response; positive regulation of endothelial cell proliferation; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling

Research Articles on NR4A1

1. The NR4A1 receptor is pro-oncogenic, regulates the ROS/endoplasmic reticulum stress pathways, and inactivation of the receptor represents a novel pathway for inducing cell death in pancreatic cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NR4A1 antibody

Reference Product	PubMed Publications
NR4A2 antibody	>138 publications with NR4A1 and NR4A2
POMC antibody	>42 publications with NR4A1 and POMC
MEF2D antibody	>7 publications with NR4A1 and MEF2D
BCL2 antibody	>6 publications with NR4A1 and BCL2
EP300 antibody	>4 publications with NR4A1 and EP300
AKT1 antibody	>4 publications with NR4A1 and AKT1
MED1 antibody	>1 publications with NR4A1 and MED1
NR0B2 antibody	>1 publications with NR4A1 and NR0B2

Pathways associated with anti-NR4A1 antibody

Products by Pathway	Pathway Diagram
AKT Phosphorylates Targets In The Nucleus Pathway antibodies	AKT Phosphorylates Targets In The Nucleus Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies	Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
Corticotropin-releasing Hormone Pathway antibodies	Corticotropin-releasing Hormone Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Downstream Signal Transduction Pathway antibodies	Downstream Signal Transduction Pathway Diagram
Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies	Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
Downstream Signaling Of Activated FGFR Pathway antibodies	Downstream Signaling Of Activated FGFR Pathway Diagram

Diseases associated with anti-NR4A1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>63 publications with NR4A1 and Inflammation
Cardiovascular Diseases Antibodies	>62 publications with NR4A1 and Cardiovascular Diseases
Nervous System Diseases Antibodies	>52 publications with NR4A1 and Nervous System Diseases
Brain Diseases Antibodies	>44 publications with NR4A1 and Brain Diseases
Necrosis Antibodies	>39 publications with NR4A1 and Necrosis
Disease Models, Animal Antibodies	>36 publications with NR4A1 and Disease Models, Animal
Atherosclerosis Antibodies	>30 publications with NR4A1 and Atherosclerosis
Lung Diseases Antibodies	>18 publications with NR4A1 and Lung Diseases
Liver Diseases Antibodies	>17 publications with NR4A1 and Liver Diseases
Breast Neoplasms Antibodies	>16 publications with NR4A1 and Breast Neoplasms

Organs/Tissues associated with anti-NR4A1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>186 publications with NR4A1 and Brain
Blood Antibodies	>175 publications with NR4A1 and Blood
Nerve Antibodies	>168 publications with NR4A1 and Nerve
Thyroid Antibodies	>125 publications with NR4A1 and Thyroid
Muscle Antibodies	>81 publications with NR4A1 and Muscle
Liver Antibodies	>54 publications with NR4A1 and Liver
Thymus Antibodies	>40 publications with NR4A1 and Thymus
Adrenal Gland Antibodies	>40 publications with NR4A1 and Adrenal Gland
Testis Antibodies	>33 publications with NR4A1 and Testis
Lung Antibodies	>32 publications with NR4A1 and Lung