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ATG13 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224327

MBS224327 recognises human Autophagy-related protein 13 (ATG13), a 517 amino acid protein belonging to the ATG13 metazoan family. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ATG13, Polyclonal Antibody
Full Product Name
RABBIT ANTI ATG13
Product Gene Name
anti-ATG13 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
This item recognises human Autophagy-related protein 13 (ATG13), a 517 amino acid protein belonging to the ATG13 metazoan family.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life: 18 months from date of despatch.
Molecular Weight
60,155 Da
OMIM
615088
3D Structure
ModBase 3D Structure for O75143
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Preparation||Antisera to Human ATG13 were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Immunogen
A synthetic peptide corresponding to 15 amino acid sequence from near the carboxy terminus of human ATG13
Histology Positive Control Tissue||Mouse Heart
Buffer Solution||Phosphate buffered saline.
Target Species
Human
Applications Tested/Suitable for anti-ATG13 antibody
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-ATG13 antibody
ELISA: This product is suitable for use in indirect ELISA applications.
Western Blot: This item detects a band of approximately 49 kDa in Rat heart tissue lysates.
Immunohistology - Paraffin: Minimum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1ug/ml

Testing Data #1 of anti-ATG13 antibody

Formalin fixed, paraffin embedded mouse heart stained with Rabbit anti Human ATG13 (MBS224327) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Rabbit Anti-ATG13 Polyclonal Antibody – Cat# MBS224327

Testing Data #2 of anti-ATG13 antibody

Western blot analysis of rat heart tissue lysate probed with Rabbit anti Mouse ATG13 (MBS224327) at 1μgml-1 in the absence (A) or presence (B) of blocking peptide
Rabbit Anti-ATG13 Polyclonal Antibody, Testing Data #2 – Cat# MBS224327

Other Notes

Small volumes of anti-ATG13 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG13 purchase

MBS224327 is an antibody targeting ATG13. Also known as RABBIT ANTI ATG13. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG13. It may not necessarily be applicable to this product.

NCBI GI #
218082953
NCBI GeneID
9776
NCBI Accession #
NP_001136145.1
UniProt Primary Accession #
O75143
UniProt Secondary Accession #
Q53EN6; Q9BRL3; Q9H8B0; B4DFI4; D3DQQ1; D3DQQ2; E9PQZ8
UniProt Related Accession #
O75143
GenBank Nucleotide #
NM_001142673.2
NCBI Official Full Name
autophagy-related protein 13 isoform 1
NCBI Official Synonym Full Names
autophagy related 13
NCBI Official Symbol
ATG13
NCBI Official Synonym Symbols
KIAA0652; PARATARG8
UniProt Protein Name
Autophagy-related protein 13
Protein Family
Autophagy protein
UniProt Gene Name
ATG13
UniProt Synonym Gene Names
KIAA0652
UniProt Entry Name
ATG13_HUMAN
NCBI Protein Information
autophagy-related protein 13; ATG13 autophagy related 13 homolog

UniProt Comments for ATG13

ATG13: an autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. Phosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Part of a complex consisting of ATG13, ULK1 and RB1CC1. Interacts with ATG101. Interacts with ULK1 and -2 via C-terminus. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Vesicle

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: mitochondrion; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: autophagic vacuole formation
Show more

Research Articles on ATG13

1. Specific affinity of the LC3 isoforms to the Atg13 LC3-interacting region is required for proper autophagosome formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ATG13 antibody

 Products by Pathway  Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram

Diseases associated with anti-ATG13 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >5 publications with ATG13 and Kidney Diseases
 Neurologic Manifestations Antibodies  >2 publications with ATG13 and Neurologic Manifestations
 Nervous System Diseases Antibodies  >2 publications with ATG13 and Nervous System Diseases
 Breast Neoplasms Antibodies  >2 publications with ATG13 and Breast Neoplasms
 Embryo Loss Antibodies  >1 publications with ATG13 and Embryo Loss
 Cell Transformation, Neoplastic Antibodies  >1 publications with ATG13 and Cell Transformation, Neoplastic
 Hypertrophy Antibodies  >1 publications with ATG13 and Hypertrophy
 Atrophy Antibodies  >1 publications with ATG13 and Atrophy
 Necrosis Antibodies  >1 publications with ATG13 and Necrosis

Organs/Tissues associated with anti-ATG13 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >5 publications with ATG13 and Kidney
 Vascular Antibodies  >4 publications with ATG13 and Vascular
 Muscle Antibodies  >4 publications with ATG13 and Muscle
 Blood Antibodies  >2 publications with ATG13 and Blood
 Salivary Gland Antibodies  >1 publications with ATG13 and Salivary Gland
 Liver Antibodies  >1 publications with ATG13 and Liver
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