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CD8 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224414

Rat anti Dog CD8 antibody, clone YCATE55.9 was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobbold et al. 1994). YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8alpha (Gorman et al. 1994) and blocks MHC… More details

$500 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD8, Monoclonal Antibody
Full Product Name
RAT ANTI DOG CD8
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
YCATE55.9
Host
Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
26,036 Da
3D Structure
ModBase 3D Structure for P33706
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Dog
Carrier Free
Yes
Immunogen
Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.
Applications Tested/Suitable for anti-CD8 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-CD8 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100

Testing Data #1 of anti-CD8 antibody

Staining of canine peripheral blood lymphocytes with Rat anti Canine CD8:FITC (MBS214752)
Rat Anti-CD8 Monoclonal Antibody – Cat# MBS224414

Testing Data #2 of anti-CD8 antibody

Staining of canine peripheral blood lymphocytes with Rat anti Canine CD8:RPE (MBS214753)
Rat Anti-CD8 Monoclonal Antibody, Testing Data #2 – Cat# MBS224414

Other Notes

Small volumes of anti-CD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD8 purchase

MBS224414 is an antibody targeting CD8. Also known as RAT ANTI DOG CD8. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain
NCBI Official Symbol
CD8A
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Synonym Protein Names
CD_antigen: CD8a
Protein Family
UniProt Gene Name
CD8A
UniProt Entry Name
CD8A_CANFA
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain; CD8 antigen, alpha polypeptide (p32)

UniProt Comments for CD8

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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