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CD68 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224483

MBS224483 is an antibody targeting CD68. Also known as RAT ANTI MOUSE CD68:Endotoxin Low. Host: Rat. More details

$900 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD68, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD68:Endotoxin Low
Product Gene Name
anti-CD68 antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
FA-11
Host
Rat
Form/Format
Low Endotoxin
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
33,844 Da
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Purified Concanavalin A acceptor glycoprotein from P815 cell line
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD68 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)*
Application Notes for anti-CD68 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilization is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Immunohistology - Paraffin: Application Note: This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Martin-Manso for details. Staining has also been achieved without antigen retrieval, see Lu for details.
Western Blotting: Application Note: Non-reducing conditions recommended.

Testing Data #1 of anti-CD68 antibody

Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:Biotin (MBS215398).following permeablisation with Leucoperm
Rat Anti-CD68 Monoclonal Antibody – Cat# MBS224483

Testing Data #2 of anti-CD68 antibody

Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:FITC (MBS215400). following permeablisation with Leucoperm
Rat Anti-CD68 Monoclonal Antibody, Testing Data #2 – Cat# MBS224483

Testing Data #3 of anti-CD68 antibody

Staining of permeabilised mouse peritoneal macrophages cells with Rat anti Mouse CD68 (MBS215395) visualised with F(ab')2 Goat anti Rat IgG:FITC (Mouse adsorbed) (MBS235148)
Rat Anti-CD68 Monoclonal Antibody, Testing Data #3 – Cat# MBS224483

Testing Data #4 of anti-CD68 antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse CD68 CD68 (MBS215403), following permeablisation with Leucoperm
Rat Anti-CD68 Monoclonal Antibody, Testing Data #4 – Cat# MBS224483

Testing Data #5 of anti-CD68 antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa FluorR 488 (MBS215395). following permeablisation with Leucoperm
Rat Anti-CD68 Monoclonal Antibody, Testing Data #5 – Cat# MBS224483

Testing Data #6 of anti-CD68 antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: RPE (MBS215405). following permeablisation with Leucoperm
Rat Anti-CD68 Monoclonal Antibody, Testing Data #6 – Cat# MBS224483

Testing Data #7 of anti-CD68 antibody

Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa FluorR 647 (MBS215395). following permeablisation with Leucoperm
Rat Anti-CD68 Monoclonal Antibody, Testing Data #7 – Cat# MBS224483

Testing Data #8 of anti-CD68 antibody

Staining of mouse spleen cryosection with Rat anti Mouse CD68 (MBS215395) followed by Goat anti Rat IgG:HRP (MBS220363) showing staining of macrophages in the red pulp
Rat Anti-CD68 Monoclonal Antibody, Testing Data #8 – Cat# MBS224483

Testing Data #9 of anti-CD68 antibody

Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 (MBS215403) with Goat anti Rat IgG:HRP MBS235195) as a detection antibody
Rat Anti-CD68 Monoclonal Antibody, Testing Data #9 – Cat# MBS224483

Other Notes

Small volumes of anti-CD68 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD68. It may not necessarily be applicable to this product.

NCBI GI #
6753352
NCBI GeneID
12514
NCBI Accession #
NP_033983.1
UniProt Secondary Accession #
O54688; O70321; Q3S4A9; Q5F2A8; Q9DD15
UniProt Related Accession #
P31996
GenBank Nucleotide #
NM_009853.1
NCBI Official Synonym Full Names
CD68 antigen
NCBI Official Symbol
Cd68
NCBI Official Synonym Symbols
Lamp4; gp110; Scard1
UniProt Protein Name
Macrosialin
UniProt Synonym Protein Names
CD_antigen: CD68
Protein Family
Macrosialin
UniProt Gene Name
Cd68
UniProt Entry Name
CD68_MOUSE
NCBI Protein Information
macrosialin

UniProt Comments for CD68

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: membrane; lysosome; integral to membrane; plasma membrane; endosome
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Research Articles on CD68

1. Statins promote the beneficial remodeling of plaques in diseased mouse arteries through the stimulation of the CCR7 / CD68 emigration pathway in macrophages

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD68 antibody

 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Macrophage Markers Pathway antibodies  Macrophage Markers Pathway Diagram

Diseases associated with anti-CD68 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1681 publications with CD68 and Inflammation
 Nervous System Diseases Antibodies  >1021 publications with CD68 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >936 publications with CD68 and Cardiovascular Diseases
 Necrosis Antibodies  >906 publications with CD68 and Necrosis
 Fibrosis Antibodies  >487 publications with CD68 and Fibrosis
 Disease Models, Animal Antibodies  >468 publications with CD68 and Disease Models, Animal
 Lung Diseases Antibodies  >453 publications with CD68 and Lung Diseases
 Kidney Diseases Antibodies  >379 publications with CD68 and Kidney Diseases
 Atherosclerosis Antibodies  >353 publications with CD68 and Atherosclerosis
 Liver Diseases Antibodies  >311 publications with CD68 and Liver Diseases

Organs/Tissues associated with anti-CD68 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1075 publications with CD68 and Muscle
 Skin Antibodies  >916 publications with CD68 and Skin
 Bone Antibodies  >864 publications with CD68 and Bone
 Lymph Node Antibodies  >547 publications with CD68 and Lymph Node
 Liver Antibodies  >541 publications with CD68 and Liver
 Lung Antibodies  >509 publications with CD68 and Lung
 Kidney Antibodies  >502 publications with CD68 and Kidney
 Connective Tissue Antibodies  >437 publications with CD68 and Connective Tissue
 Joint Antibodies  >387 publications with CD68 and Joint
 Heart Antibodies  >377 publications with CD68 and Heart
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