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MHC CLASS II Dq (HLA-DQA1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224521

MBS224487 specifically recognises Bovine MHC Class II DQ. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of… More details

$795 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MHC CLASS II DQ (HLA-DQA1), Monoclonal Antibody
Full Product Name
MOUSE ANTI BOVINE MHC CLASS II DQ:RPE
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
CC158
Host
Mouse
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Specificity
This item specifically recognises Bovine MHC Class II DQ. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.

The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
27,859 Da
Reconstitution
Reconstitute with 1.0ml distilled water
Perservative Stabilisers||0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Bovine
Applications Tested/Suitable for anti-HLA-DQA1 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-HLA-DQA1 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat

Testing Data #1 of anti-HLA-DQA1 antibody

Bovine peripheral blood lymphocytes stained with Mouse anti Bovine MHC Class II DQ (MBS224487) followed by Goat anti Mouse IgG:FITC (MBS235142)
Mouse Anti-HLA-DQA1 Monoclonal Antibody – Cat# MBS224521

Testing Data #2 of anti-HLA-DQA1 antibody

Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DQ:FITC (MBS224650)
Mouse Anti-HLA-DQA1 Monoclonal Antibody, Testing Data #2 – Cat# MBS224521

Testing Data #3 of anti-HLA-DQA1 antibody

Bovine peripheral blood lymphocytes stained with Mouse anti Bovine MHC Class II DQ:RPE (MBS224521)
Mouse Anti-HLA-DQA1 Monoclonal Antibody, Testing Data #3 – Cat# MBS224521

Other Notes

Small volumes of anti-HLA-DQA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HLA-DQA1 purchase

MBS224521 is an antibody targeting HLA-DQA1. Also known as MOUSE ANTI BOVINE MHC CLASS II DQ:RPE. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HLA-DQA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
MHC class II DQA2 (Major histocompatibility complex, class II, DQ alpha 1)
UniProt Protein Name
MHC class II DQA2
UniProt Synonym Protein Names
MHC class II DQA2
UniProt Gene Name
HLA-DQA1
UniProt Entry Name
Q30308_BOVIN

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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