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SIGLEC-5/SIGLEC-14 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224544

MBS224725 recognises human Siglec-5 (Sialic acid-binding Ig-like lectin 5), otherwise known as CD170, a novel sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and… More details

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Product Overview

Online Datasheet in PDF
Product Name
SIGLEC-5/SIGLEC-14, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN SIGLEC-5/SIGLEC-14:RPE
Product Gene Name
anti-SIGLEC-5/SIGLEC-14 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1A5
Host
Mouse
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Specificity
Clone 1A5 recognises human Siglec-5 (Sialic acid-binding Ig-like lectin 5), otherwise known as CD170, a novel sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage.

Clone 1A5, is one of several Siglec-5 antibodies which also recognises human Siglec-14 (Sialic acid-binding Ig-like lectin 14) see Angata, T. et al. for details. Siglec-14 shares an almost identical sequence with Siglec-5 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species.

Siglec-5 is highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), and mediates sialic-acid dependent binding to cells, as well as acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases.Siglec-14 is a putative sialic-acid binding adhesion molecule, and member of the Ig superfamily, predominantly expressed in hematopoietic tissues, which has been shown to associate with the activating adapter protein DAP12.

Clone 1A5 has been show to cross react with Chimpanzee.
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
60,715 Da
OMIM
604200
Reconstitution
Reconstitute with 1.0ml distilled water
Perservative Stabilisers||0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Siglec-5-Fc protein, consisting of the full-length extracellular region of human Siglec-5, fused with the Fc region of human IgG1.
Buffer Solution||Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-SIGLEC-5/SIGLEC-14 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-SIGLEC-5/SIGLEC-14 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells.
Flow Cytometry: Minimum Dilution: Neat

Testing Data #1 of anti-SIGLEC-5/SIGLEC-14 antibody

Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14 (MBS224725) followed by rabbit F(ab')2 anti mouse IgG:FITC (MBS235160)
Mouse Anti-SIGLEC-5/SIGLEC-14 Monoclonal Antibody – Cat# MBS224544

Testing Data #2 of anti-SIGLEC-5/SIGLEC-14 antibody

Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14:FITC (MBS224673)
Mouse Anti-SIGLEC-5/SIGLEC-14 Monoclonal Antibody, Testing Data #2 – Cat# MBS224544

Testing Data #3 of anti-SIGLEC-5/SIGLEC-14 antibody

Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14:RPE (MBS224544)
Mouse Anti-SIGLEC-5/SIGLEC-14 Monoclonal Antibody, Testing Data #3 – Cat# MBS224544

Other Notes

Small volumes of anti-SIGLEC-5/SIGLEC-14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SIGLEC-5/SIGLEC-14 purchase

MBS224544 is an antibody targeting SIGLEC-5/SIGLEC-14. Also known as MOUSE ANTI HUMAN SIGLEC-5/SIGLEC-14:RPE. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SIGLEC-5/SIGLEC-14. It may not necessarily be applicable to this product.

NCBI GI #
4502659
NCBI GeneID
8778
NCBI Accession #
NP_003821.1
UniProt Related Accession #
O15389
GenBank Nucleotide #
NM_003830.3
NCBI Official Full Name
sialic acid-binding Ig-like lectin 5
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 5
NCBI Official Symbol
SIGLEC5
NCBI Official Synonym Symbols
CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
UniProt Protein Name
Sialic acid-binding Ig-like lectin 5
UniProt Synonym Protein Names
CD33 antigen-like 2; Obesity-binding protein 2; OB-BP2; OB-binding protein 2; CD_antigen: CD170
UniProt Gene Name
SIGLEC5
UniProt Synonym Gene Names
CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2
UniProt Entry Name
SIGL5_HUMAN
NCBI Protein Information
sialic acid-binding Ig-like lectin 5; CD33 antigen-like 2; OB-binding protein 2; obesity-binding protein 2; sialic acid-binding immunoglobulin-like lectin 5

NCBI Summary for SIGLEC-5/SIGLEC-14

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]
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UniProt Comments for SIGLEC-5/SIGLEC-14

SIGLEC5: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to membrane

Molecular Function: carbohydrate binding

Biological Process: cell adhesion
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Research Articles on SIGLEC-5/SIGLEC-14

1. Data indicate that the Siglec-5/14 genotype influences neutrophil responses to group B Streptococcus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SIGLEC-5/SIGLEC-14 antibody

 Reference Product  PubMed Publications
 CD24 antibody  >1 publications with SIGLEC-5/SIGLEC-14 and CD24
 TYROBP antibody  >1 publications with SIGLEC-5/SIGLEC-14 and TYROBP

Diseases associated with anti-SIGLEC-5/SIGLEC-14 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >16 publications with SIGLEC-5/SIGLEC-14 and Inflammation
 Asthma Antibodies  >10 publications with SIGLEC-5/SIGLEC-14 and Asthma
 Disease Models, Animal Antibodies  >7 publications with SIGLEC-5/SIGLEC-14 and Disease Models, Animal
 Respiratory Hypersensitivity Antibodies  >6 publications with SIGLEC-5/SIGLEC-14 and Respiratory Hypersensitivity
 Necrosis Antibodies  >4 publications with SIGLEC-5/SIGLEC-14 and Necrosis
 Fibrosis Antibodies  >3 publications with SIGLEC-5/SIGLEC-14 and Fibrosis
 Hypertension Antibodies  >1 publications with SIGLEC-5/SIGLEC-14 and Hypertension
 Birth Weight Antibodies  >1 publications with SIGLEC-5/SIGLEC-14 and Birth Weight
 Genital Diseases, Male Antibodies  >1 publications with SIGLEC-5/SIGLEC-14 and Genital Diseases, Male
 Weight Loss Antibodies  >1 publications with SIGLEC-5/SIGLEC-14 and Weight Loss

Organs/Tissues associated with anti-SIGLEC-5/SIGLEC-14 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >41 publications with SIGLEC-5/SIGLEC-14 and Blood
 Lung Antibodies  >12 publications with SIGLEC-5/SIGLEC-14 and Lung
 Bone Antibodies  >10 publications with SIGLEC-5/SIGLEC-14 and Bone
 Bone Marrow Antibodies  >10 publications with SIGLEC-5/SIGLEC-14 and Bone Marrow
 Spleen Antibodies  >4 publications with SIGLEC-5/SIGLEC-14 and Spleen
 Kidney Antibodies  >3 publications with SIGLEC-5/SIGLEC-14 and Kidney
 Intestine Antibodies  >2 publications with SIGLEC-5/SIGLEC-14 and Intestine
 Lymph Node Antibodies  >1 publications with SIGLEC-5/SIGLEC-14 and Lymph Node
 Thymus Antibodies  >1 publications with SIGLEC-5/SIGLEC-14 and Thymus
 Brain Antibodies  >1 publications with SIGLEC-5/SIGLEC-14 and Brain
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