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TIM-3 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224586

Rat anti Mouse Tim-3 antibody, clone RMT3-23 specifically recognizes mouse T-cell immunoglobulin mucin 3, a transmembrane glycoprotein and member of the immunoglobulin superfamily, preferentially expressed by differentiated CD4+ Th1 cells and CD8+ Tc1… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TIM-3, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE TIM-3:FITC
Product Gene Name
anti-TIM-3 antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
RMT3-23
Host
Rat
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
30,934 Da
3D Structure
ModBase 3D Structure for Q8VIM0
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Tim-3-Ig, consisting of amino acids 1-191 of mouse Tim-3, coupled with the Fc portion of mouse IgG2a.
Fusion Partners||Lymph node cells from immunised SD rats were fused with cells of the P3U1 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-TIM-3 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-TIM-3 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data #1 of anti-TIM-3 antibody

Tim-3 transfected LY5178Y cells stained with Rat anti Mouse TIM-3:FITC (MBS224586)
Rat Anti-TIM-3 Monoclonal Antibody – Cat# MBS224586

Testing Data #2 of anti-TIM-3 antibody

Tim-3 transfected LY5178Y cells stained with Rat anti Mouse TIM-3 (MBS224457)
Rat Anti-TIM-3 Monoclonal Antibody, Testing Data #2 – Cat# MBS224586

Testing Data #3 of anti-TIM-3 antibody

Tim-3 transfected LY5178Y cells stained with Rat anti Mouse TIM-3:RPE (MBS224712)
Rat Anti-TIM-3 Monoclonal Antibody, Testing Data #3 – Cat# MBS224586

Testing Data #4 of anti-TIM-3 antibody

Tim-3 transfected LY5178Y cells stained with Rat anti Mouse TIM-3:Low Endotoxin(MBS2130980EL)
Rat Anti-TIM-3 Monoclonal Antibody, Testing Data #4 – Cat# MBS224586

Other Notes

Small volumes of anti-TIM-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIM-3 purchase

MBS224586 is an antibody targeting TIM-3. Also known as RAT ANTI MOUSE TIM-3:FITC. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for TIM-3. It may not necessarily be applicable to this product.

NCBI GI #
160333699
NCBI GeneID
171285
NCBI Accession #
NP_599011.2
UniProt Primary Accession #
Q8VIM0
UniProt Related Accession #
Q8VIM0
GenBank Nucleotide #
NM_134250.2
NCBI Official Full Name
hepatitis A virus cellular receptor 2 homolog
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 2
NCBI Official Symbol
Havcr2
NCBI Official Synonym Symbols
Tim3; TIM-3; Timd3
UniProt Protein Name
Hepatitis A virus cellular receptor 2 homolog
UniProt Synonym Protein Names
T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3
Protein Family
Hepatitis A virus cellular receptor
UniProt Gene Name
Havcr2
UniProt Synonym Gene Names
Tim3; Timd3; HAVcr-2; TIMD-3; TIM-3
UniProt Entry Name
HAVR2_MOUSE
NCBI Protein Information
hepatitis A virus cellular receptor 2 homolog; HAVcr-2; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIMD-3

UniProt Comments for TIM-3

TIM-3: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Belongs to the immunoglobulin superfamily. TIM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: cell surface; membrane; integral to membrane

Molecular Function: protein binding
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Research Articles on TIM-3

1. The TIM-3 pathway ameliorates Theiler's murine encephalomyelitis virus-induced demyelinating disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TIM-3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >53 publications with TIM-3 and Inflammation
 Disease Models, Animal Antibodies  >43 publications with TIM-3 and Disease Models, Animal
 Lung Diseases Antibodies  >31 publications with TIM-3 and Lung Diseases
 Liver Diseases Antibodies  >30 publications with TIM-3 and Liver Diseases
 Necrosis Antibodies  >26 publications with TIM-3 and Necrosis
 Asthma Antibodies  >23 publications with TIM-3 and Asthma
 Respiratory Hypersensitivity Antibodies  >21 publications with TIM-3 and Respiratory Hypersensitivity
 Neoplasms, Experimental Antibodies  >20 publications with TIM-3 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >15 publications with TIM-3 and Cardiovascular Diseases
 Skin Diseases Antibodies  >14 publications with TIM-3 and Skin Diseases

Organs/Tissues associated with anti-TIM-3 antibody

 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >21 publications with TIM-3 and Spleen
 Brain Antibodies  >7 publications with TIM-3 and Brain
 Pancreas Antibodies  >2 publications with TIM-3 and Pancreas
 Uterus Antibodies  >1 publications with TIM-3 and Uterus
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