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CD27 Antibody – Hamster Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224636

Clone LG.3A10 recognises mouse, rat and human CD27, a member of the tumour necrosis factor receptor superfamily. It is expressed in the thymus and spleen, but not in non-lymphoid tissues. CD27 acts as a ligand for CD70, which is expressed in T and B cells,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD27, Monoclonal Antibody
Full Product Name
HAMSTER ANTI MOUSE CD27:Biotin
Product Gene Name
Clonality
Monoclonal
Isotype
IgG
Clone Number
LG.3A10
Host
Hamster
Form/Format
Biotin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.5mg/ml (lot specific)
Specificity
Clone LG.3A10 recognises mouse, rat and human CD27, a member of the tumour necrosis factor receptor superfamily. It is expressed in the thymus and spleen, but not in non-lymphoid tissues. CD27 acts as a ligand for CD70, which is expressed in T and B cells, and acts as a costimulatory signal in lymphocyte proliferation and immunoglobulin production. CD27 lacks a death domain in its short cytoplasmic tail, but can induce apoptosis by binding with the apoptosis regulatory protein Siva (also known as CD27-binding protein).
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation||Purified IgG prepared by affinity chromatography
Immunogen
Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD27 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes for anti-CD27 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/20; Maximum Dilution: 1/50

Other Notes

Small volumes of anti-CD27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD27 purchase

MBS224636 is an antibody targeting CD27. Also known as HAMSTER ANTI MOUSE CD27:Biotin. Host: Hamster.

Additional Details

NCBI/Uniprot data below describe general gene information for CD27. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 molecule
NCBI Official Symbol
Cd27
NCBI Official Synonym Symbols
Tnfrsf7
Protein Family
NCBI Protein Information
CD27 antigen; CD antigen 27; tumor necrosis factor receptor superfamily, member 7

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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