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CLASS II Monomorphic (MHC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224644

Mouse anti Bovine MHC Class II Monomorphic antibody, clone IL-A21 recognizes a monomorphic epitope within the bovine MHC II cell surface antigen. Clone IL-A21 is reported to react with an epitope which is common to both BoLA DR and DQ  (Howard et al.1997).… More details

$795 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MHC CLASS II MONOMORPHIC, Monoclonal Antibody
Full Product Name
MOUSE ANTI BOVINE MHC CLASS II MONOMORPHIC:RPE
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
IL-A21
Host
Mouse
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Reconstitution
Reconstitute with 1.0 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Bovine
Applications Tested/Suitable for anti-MHC CLASS II MONOMORPHIC antibody
Flow cytometry (FC/FACS)
Application Notes for anti-MHC CLASS II MONOMORPHIC antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat

Testing Data #1 of anti-MHC CLASS II MONOMORPHIC antibody

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic) (MBS224486)
Mouse Anti-MHC Monoclonal Antibody – Cat# MBS224644

Testing Data #2 of anti-MHC CLASS II MONOMORPHIC antibody

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic):FITC (MBS224721)
Mouse Anti-MHC Monoclonal Antibody, Testing Data #2 – Cat# MBS224644

Testing Data #3 of anti-MHC CLASS II MONOMORPHIC antibody

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic):RPE (MBS224644)
Mouse Anti-MHC Monoclonal Antibody, Testing Data #3 – Cat# MBS224644

Other Notes

Small volumes of anti-MHC CLASS II MONOMORPHIC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MHC CLASS II MONOMORPHIC purchase

MBS224644 is an antibody targeting MHC CLASS II MONOMORPHIC. Also known as MOUSE ANTI BOVINE MHC CLASS II MONOMORPHIC:RPE. Host: Mouse.
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