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CD39 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224685

Rat anti Mouse CD39 antibody, clone Duha59 recognizes CD39, also known as Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or Ecto-ATP diphosphohydrolase. CD39 was first described as a B cell activation marker, but is also expressed on natural killer… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD39, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD39:RPE
Product Gene Name
anti-CD39 antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
Duha59
Host
Rat
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
57,205 Da
3D Structure
ModBase 3D Structure for P55772
Reconstitution
Reconstitute with 1.0ml distilled water
Perservative Stabilisers||0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
A CD39 expression construct and a final boost with CD39-transfected HEK cells.
Fusion Partners||Spleen cells from immunised rats were fused with cells of the Sp2/0 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD39 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD39 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Testing Data #1 of anti-CD39 antibody

Mouse peritoneal macrophages stained with Rat anti Mouse CD39:Alexa FluorR -647 (MBS219376)
Rat Anti-CD39 Monoclonal Antibody – Cat# MBS224685

Testing Data #2 of anti-CD39 antibody

Mouse peritoneal macrophages stained with Rat anti Mouse CD39:FITC (MBS219599)
Rat Anti-CD39 Monoclonal Antibody, Testing Data #2 – Cat# MBS224685

Testing Data #3 of anti-CD39 antibody

Mouse peritoneal macrophages stained with Rat anti Mouse CD39 (MBS219376)
Rat Anti-CD39 Monoclonal Antibody, Testing Data #3 – Cat# MBS224685

Testing Data #4 of anti-CD39 antibody

Mouse peritoneal macrophages stained with Rat anti Mouse CD39:RPE (MBS224685)
Rat Anti-CD39 Monoclonal Antibody, Testing Data #4 – Cat# MBS224685

Other Notes

Small volumes of anti-CD39 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD39 purchase

MBS224685 is an antibody targeting CD39. Also known as RAT ANTI MOUSE CD39:RPE. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD39. It may not necessarily be applicable to this product.

NCBI GI #
6753346
NCBI GeneID
12495
NCBI Accession #
NP_033978.1
UniProt Primary Accession #
P55772
UniProt Related Accession #
P55772
GenBank Nucleotide #
NM_009848.4
NCBI Official Full Name
ectonucleoside triphosphate diphosphohydrolase 1 isoform 2
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 1
NCBI Official Symbol
Entpd1
NCBI Official Synonym Symbols
Cd39; AA408691; NTPDase-1; 2610206B08Rik
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 1
UniProt Synonym Protein Names
Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen; CD_antigen: CD39
Protein Family
Ectonucleoside triphosphate diphosphohydrolase
UniProt Gene Name
Entpd1
UniProt Synonym Gene Names
Cd39; NTPDase 1; Ecto-ATPDase 1; Ecto-ATPase 1
UniProt Entry Name
ENTP1_MOUSE
NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 1; CD39 antigen; NTPDase 1; ecto-ATP diphosphohydrolase 1; ecto-ATPDase 1; ecto-ATPase 1; ectoapyrase; lymphoid cell activation antigen

UniProt Comments for CD39

ENTPD1: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Belongs to the GDA1/CD39 NTPase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Extracellular matrix; Membrane protein, integral; Hydrolase; Membrane protein, multi-pass; EC 3.6.1.5; Nucleotide Metabolism - pyrimidine

Cellular Component: extracellular space; synaptic vesicle; neuron projection; cell surface; cell soma; membrane; basolateral plasma membrane; postsynaptic density; integral to membrane; basal lamina

Molecular Function: identical protein binding; hydrolase activity; nucleoside-diphosphatase activity; ATPase activity; nucleotide binding; nucleoside-triphosphatase activity; ATP binding; adenosine-diphosphatase activity

Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; metabolic process; purine ribonucleoside diphosphate catabolic process; negative regulation of dopamine secretion; protein homooligomerization
Show more

Research Articles on CD39

1. NTPDase1 controls male fertility via the regulation of P2X1 activation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD39 antibody

 Products by Pathway  Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram

Diseases associated with anti-CD39 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >13 publications with CD39 and Inflammation
 Disease Models, Animal Antibodies  >8 publications with CD39 and Disease Models, Animal
 Necrosis Antibodies  >5 publications with CD39 and Necrosis
 Liver Diseases Antibodies  >3 publications with CD39 and Liver Diseases
 Kidney Diseases Antibodies  >3 publications with CD39 and Kidney Diseases
 Liver Neoplasms Antibodies  >3 publications with CD39 and Liver Neoplasms
 Nervous System Diseases Antibodies  >2 publications with CD39 and Nervous System Diseases
 Atherosclerosis Antibodies  >1 publications with CD39 and Atherosclerosis
 Carcinoma, Hepatocellular Antibodies  >1 publications with CD39 and Carcinoma, Hepatocellular
 Hypertension Antibodies  >1 publications with CD39 and Hypertension

Organs/Tissues associated with anti-CD39 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >22 publications with CD39 and Blood
 Kidney Antibodies  >4 publications with CD39 and Kidney
 Pancreas Antibodies  >3 publications with CD39 and Pancreas
 Bone Marrow Antibodies  >3 publications with CD39 and Bone Marrow
 Brain Antibodies  >2 publications with CD39 and Brain
 Skin Antibodies  >2 publications with CD39 and Skin
 Embryonic Tissue Antibodies  >2 publications with CD39 and Embryonic Tissue
 Artery Antibodies  >2 publications with CD39 and Artery
 Lung Antibodies  >1 publications with CD39 and Lung
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