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CD25 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224689

Mouse anti Chicken CD25 monoclonal antibody, clone AV142 specifically recognizes CD25, the chicken IL-2 Receptor alpha chain (IL-2Ralpha) which has been identified as the chicken homologue of human CD25 and is cited as ChCD25 in published literature. Chicken… More details

$460 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD25, Monoclonal Antibody
Full Product Name
MOUSE ANTI CHICKEN CD25
Product Gene Name
anti-CD25 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AV142
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Perservative Stabilisers
<0.1% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Chicken CD25/hulg fusion protein
Buffer Solution
Phosphate buffered saline
Target Species
Chicken
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 Ag14 Myeloma cell line.
Applications Tested/Suitable for anti-CD25 antibody
ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-CD25 antibody
ELISA: This product is suitable for use in direct ELISA applications.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Testing Data #1 of anti-CD25 antibody

ELISA analysis of recombinant chicken CD25 (supernatant from transfected COS cells, 1/3 dilution) with Mouse anti Chicken CD25 antibody (AV142). Supernatant from mock-transfected COS cells was used as negative control (Image courtesy of the Roslin Institute.)
Mouse Anti-CD25 Monoclonal Antibody – Cat# MBS224689

Testing Data #2 of anti-CD25 antibody

Western blot analysis of chicken CD25/huIg fusion protein with Mouse anti Chicken CD25 (AV142). Recombinant huIg was used as negative control (Image courtesy of the Roslin Institute.)
Mouse Anti-CD25 Monoclonal Antibody, Testing Data #2 – Cat# MBS224689

Testing Data #3 of anti-CD25 antibody

Staining of ConA-stimulated chicken splenocytes with Mouse anti Chicken CD25 (AV142) conjugated with Alexa fluor-647
(Image courtesy of the Roslin Institute.)
Mouse Anti-CD25 Monoclonal Antibody, Testing Data #3 – Cat# MBS224689

Other Notes

Small volumes of anti-CD25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD25 purchase

MBS224689 is an antibody targeting CD25. Also known as MOUSE ANTI CHICKEN CD25. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD25. It may not necessarily be applicable to this product.

NCBI GI #
45382989
NCBI GeneID
395294
NCBI Accession #
NP_989927.1
GenBank Nucleotide #
NM_204596.1
NCBI Official Full Name
interleukin 2 receptor, alpha
NCBI Official Synonym Full Names
interleukin 2 receptor, alpha
NCBI Official Symbol
IL2RA
Protein Family
Interleukin-2 receptor
NCBI Protein Information
interleukin 2 receptor, alpha; IL-2 alpha receptor; IL2 receptor alpha; interleukin-2 alpha receptor

Research Articles on CD25

1. chCD25 molecules could be expressed on the surface of monocytes/macrophages, thrombocytes, CD4+ and CD8+ cells as well as tissue cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD25 antibody

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