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CD28 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS224710

Mouse anti Bovine CD28, clone CC219, recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Clone CC219 has been shown to be able to stimulate… More details

$705 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD28, Monoclonal Antibody
Full Product Name
MOUSE ANTI BOVINE CD28:FITC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CC219
Host
Mouse
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
Ig concentration 0.1mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
25,143 Da
3D Structure
ModBase 3D Structure for Q28071
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein A
Immunogen
Plasmid DNA encoding the bovine CD28 gene
Fusion Partners||Spleen cells from immunised Balb/c mice were fused with cells of the SP/2/0 myeloma cell line
Buffer Solution||Phosphate buffered saline
Target Species
Bovine
Applications Tested/Suitable for anti-CD28 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD28 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data #1 of anti-CD28 antibody

Bovine PBMCs stained with Mouse anti Bovine CD28 (MBS224419) followed by Goat anti Mouse IgG:FITC (MBS235142)
Mouse Anti-CD28 Monoclonal Antibody – Cat# MBS224710

Testing Data #2 of anti-CD28 antibody

Bovine PBMCs stained with Mouse anti Bovine CD28:FITC (MBS224710)
Mouse Anti-CD28 Monoclonal Antibody, Testing Data #2 – Cat# MBS224710

Other Notes

Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD28 purchase

MBS224710 is an antibody targeting CD28. Also known as MOUSE ANTI BOVINE CD28:FITC. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28
NCBI Official Synonym Full Names
CD28 molecule<
NCBI Official Symbol
CD28
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Synonym Protein Names
CD_antigen: CD28
UniProt Gene Name
CD28
UniProt Entry Name
CD28_BOVIN
NCBI Protein Information
T-cell-specific surface glycoprotein CD28; antigen CD28

UniProt Comments for CD28

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-CD28 antibody

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