CD3 Antibody – Mouse Monoclonal

Mouse anti Pig CD3, clone PPT3 recognizes the porcine homologue of human CD3epsilon, a 24 kDa single pass type I membrane protein expressed by T-lymphocytes. Clone PPT3, also known under the clone designation FY1H2, was clustered at the second international swine CD workshop and found to specifically recognise an epitope on the porcine CD3epsilon designated as CD3c (Pescovitz, M.D., et al. 1998).CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, gamma, delta, zeta) that assemble and function as three pairs of dimers (epsilongamma, epsilondelta, zetazeta). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Guy, C.S & Vignali, D.G. 2009). CD3 is a defining feature of cells belonging to the T cell lineage, antibodies recognising pig CD3 therefore provide useful markers of porcine T cells. Clone PPT3 has been demonstrated to recognise an epitope that is expressed both intracellularly and extracellularly, additionally clone PPT3 has been demonstrated to activate alpha/beta T-cells (Kirkham P.A., et al. 1996). Epitope mapping studies have demonstrated that clone PPT3 recognises an alternative epitope to clone PPT6 (Yang, H. et al. 1996; Pescovitz, M.D., et al. 1998). Clone PPT3 was tested on PBL from a range of other mammalian species and found to be negative suggesting that the epitope recognised by this clone is specific to porcine (Yang, H. et al. 1996).

Small volumes of anti-CD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS224837 is an antibody targeting CD3. Also known as MOUSE ANTI PIG CD3. Reactive in Does not react with:Bovine, Goat, Horse, Human, Sheep. Host: Mouse.