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Alpha (TNF) Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225042

MBS225042 is an antibody targeting TNF. Also known as Rabbit anti Pig TNF Alpha. Reactive in Reacts with: Dog, Bovine, Dolphin, CatN.B. Antibody reactivity and working conditions may vary between species.. RABBIT ANTI PIG TNF ALPHA More details

$810 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TNF Alpha, Polyclonal Antibody
Full Product Name
Rabbit anti Pig TNF Alpha
Product Gene Name
Product Synonym Names
TNFSF2
Clonality
Polyclonal
Isotype
Polyclonal IgG
Species Reactivity
Reacts with: Dog, Bovine, Dolphin, Cat
N.B. Antibody reactivity and working conditions may vary between species.
Form/Format
Purified; Purified IgG - liquid; Phosphate buffered saline
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Specificity
Rabbit anti Pig TNF alpha antibody recognizes porcine tumor necrosis factor alpha (TNFα). TNFα is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNFα has a key regulatory role in the porcine immune response where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.
Preparation and Storage
Store at +4°C. Do not freeze. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
3D Structure
ModBase 3D Structure for P23563
Target Species
Pig
Antiserum Preparation
Antisera to pig TNF alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant pig tumor necrosis factor alpha (TNFα)
Applications Tested/Suitable for anti-TNF antibody
Flow Cytometry (FC), Immunohistochemistry- Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes for anti-TNF antibody
IP: 1ug/ml - 5ug/ml
WB: 0.1ug/ml - 2ug/ml
ELISA This product may be used as a capture antibody in a sandwich ELISA together with MBS225043 as the detection reagent. Recombinant Pig TNF alpha (MBS225424) may be used as a standard.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA (EIA) of anti-TNF antibody

Pig TNF Alpha ELISA Recombinant Pig TNF alpha detected using Rabbit anti Pig TNF alpha as the capture reagent and Rabbit anti Pig TNF alpha:Biotin as the detection reagent followed by Streptavidin:HRP
Anti-TNF Polyclonal Antibody – Cat# MBS225042

Other Notes

Small volumes of anti-TNF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TNF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
TNFa
UniProt Protein Name
Tumor necrosis factor
UniProt Synonym Protein Names
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Protein Family
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
NCBI Protein Information
tumor necrosis factor

UniProt Comments for TNF

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-TNF antibody

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