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NOS2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225046

Rabbit anti iNOS antibody recognizes inducible nitric oxide synthase also known as iNOS and NOS type II. This enzyme triggers the synthesis of the second messenger molecule, nitric oxide (NO), by immune cells such as macrophages in response to pathogen… More details

$540 / 0.05 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
iNOS (Nos2), Polyclonal Antibody
Full Product Name
Rabbit anti iNOS
Product Gene Name
Product Synonym Names
iNOS Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat, Bovine
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3), 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography on Protein A.
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.
Molecular Weight
130,575 Da
3D Structure
ModBase 3D Structure for P29477
Immunogen
Synthetic peptide corresponding to aa 1131-1144 of mouse iNOS
Preperation
Antiserum to iNOS was raised by repeated immunisation of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-Nos2 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-Nos2 antibody
WB: 1:1000

Western Blot (WB) of anti-Nos2 antibody

Western blot analysis of rat brain extract probed with Rabbit anti iNOS
Rabbit Anti-NOS2 Polyclonal Antibody – Cat# MBS225046

Other Notes

Small volumes of anti-Nos2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nos2 purchase

MBS225046 is an antibody targeting Nos2. Also known as Rabbit anti iNOS. Reactive in Mouse, Rat, Bovine. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Nos2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Nitric oxide synthase, inducible
NCBI Official Synonym Full Names
nitric oxide synthase 2, inducible
NCBI Official Symbol
Nos2
NCBI Official Synonym Symbols
iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Synonym Protein Names
Inducible NO synthase; Inducible NOS; iNOS; Macrophage NOS; MAC-NOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
Protein Family
UniProt Gene Name
Nos2
UniProt Synonym Gene Names
Inosl; Inducible NOS; iNOS; MAC-NOS
NCBI Protein Information
nitric oxide synthase, inducible

NCBI Summary for Nos2

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
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UniProt Comments for Nos2

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7503239). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (PubMed:16373578). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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