• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DFF-45 (DFFA) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225102

Rabbit anti DFF-45 antibody recognizes DFF, also known as DNA fragmentation factor subunit alpha, DFF1, DFFA, inhibitor of caspase-activated DNase or ICAD.DNA fragmentation factor (DFF) is a heterodimeric protein of 40kDa (DFF-40) and 45 kDa (DFF-45)… More details

$540 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DFF-45 (DFFA), Polyclonal Antibody
Full Product Name
Rabbit anti DFF-45
Product Gene Name
Product Synonym Names
DFF-45 Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
29,411 Da
OMIM
601882
3D Structure
ModBase 3D Structure for O00273
Immunogen
Recombinant human DFF-45
Preperation
Antiserum to DFF-45 was raised by repeated immunization of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-DFFA antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-DFFA antibody
IHC-Paraffin: 1:50-1:200
WB: 1:500-1:2000

Western Blot (WB) of anti-DFFA antibody

Western blot analysis of cell and tissue lysates using Rabbit anti DFF-45 antibody
Rabbit Anti-DFFA Polyclonal Antibody – Cat# MBS225102

Immunohistochemistry (IHC) of anti-DFFA antibody

Immunohistochemistry of paraffin-embedded human stomach using Rabbit anti DFF-45 antibody at a dilution of 1/100
Rabbit Anti-DFFA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS225102

Immunohistochemistry (IHC) of anti-DFFA antibody

Immunohistochemistry of paraffin-embedded human stomach using Rabbit anti DFF-45 antibody at a dilution of 1/100
Rabbit Anti-DFFA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS225102

Other Notes

Small volumes of anti-DFFA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DFFA purchase

MBS225102 is an antibody targeting DFFA. Also known as Rabbit anti DFF-45. Reactive in Human, Mouse (Expected from Sequence), Rat (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DFFA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DNA fragmentation factor subunit alpha
NCBI Official Synonym Full Names
DNA fragmentation factor subunit alpha
NCBI Official Symbol
DFFA
NCBI Official Synonym Symbols
DFF1; ICAD; DFF-45
UniProt Protein Name
DNA fragmentation factor subunit alpha
UniProt Synonym Protein Names
DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD
UniProt Gene Name
DFFA
UniProt Synonym Gene Names
DFF1; DFF45; DFF-45; ICAD
NCBI Protein Information
DNA fragmentation factor subunit alpha

NCBI Summary for DFFA

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for DFFA

Inhibitor of the caspase-activated DNase (DFF40).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000