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EIF4EBP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225181

Rabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
4EBP1 (EIF4EBP1), Polyclonal Antibody
Full Product Name
Rabbit anti 4EBP1 (pSer65)
Product Gene Name
Product Synonym Names
4EBP1 (pSer65) Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
12,580 Da
OMIM
602223
3D Structure
ModBase 3D Structure for Q13541
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 65 of human 4EBP1
Preperation
Antiserum to 4EBP1 (pSer65) was raised by repeated immunization of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-EIF4EBP1 antibody
Western Blot (WB)
Application Notes for anti-EIF4EBP1 antibody
WB: 1:500-1:2000

Western Blot (WB) of anti-EIF4EBP1 antibody

Western blot analysis of extracts from HEK-293 cells using Rabbit anti 4EBP1 (pSer65) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with FBS or ?-phosphatase.
Rabbit Anti-EIF4EBP1 Polyclonal Antibody – Cat# MBS225181

Other Notes

Small volumes of anti-EIF4EBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4EBP1 purchase

MBS225181 is an antibody targeting EIF4EBP1. Also known as Rabbit anti 4EBP1 (pSer65). Reactive in Human, Mouse (Expected from Sequence), Rat (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4EBP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names
Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
UniProt Gene Name
EIF4EBP1
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1

NCBI Summary for EIF4EBP1

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
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UniProt Comments for EIF4EBP1

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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