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BCR Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225198

Rabbit anti BCR (pTyr177) antibody recognizes breakpoint cluster region protein (BCR), also known as Renal carcinoma antigen NY-REN-26, when phosphorylated at tyrosine 177. The BCR protein is a GTPase activating protein and a serine/threonine kinase. A… More details

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Technical Datasheet View PDF
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Product Overview

Product Name
BCR, Polyclonal Antibody
Full Product Name
Rabbit anti BCR (pTyr177)
Product Gene Name
Product Synonym Names
Bcr (pTyr177) Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse (Expected from Sequence)
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
137,729 Da
OMIM
151410
3D Structure
ModBase 3D Structure for P11274
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 177 of human BCR
Preperation
Antiserum to BCR (pTyr177) was raised by repeated immunization of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-BCR antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-BCR antibody
IHC-Paraffin: 1:50-1:100
WB: 1:500-1:2000

Western Blot (WB) of anti-BCR antibody

Western blot analysis of extracts from K562 cells untreated or treated with H2O2 using Rabbit anti BCR (pTyr177) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with H2O2 (2mM) for 15 minutes
Rabbit Anti-BCR Polyclonal Antibody – Cat# MBS225198

Immunohistochemistry (IHC) of anti-BCR antibody

Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue using Rabbit anti BCR (pTyr177) antibody
Rabbit Anti-BCR Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS225198

Other Notes

Small volumes of anti-BCR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BCR purchase

MBS225198 is an antibody targeting BCR. Also known as Rabbit anti BCR (pTyr177). Reactive in Human, Mouse (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BCR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Breakpoint cluster region protein
NCBI Official Synonym Full Names
BCR, RhoGEF and GTPase activating protein
NCBI Official Symbol
BCR
NCBI Official Synonym Symbols
ALL; CML; PHL; BCR1; D22S11; D22S662
UniProt Protein Name
Breakpoint cluster region protein
UniProt Synonym Protein Names
Renal carcinoma antigen NY-REN-26
UniProt Gene Name
BCR
UniProt Synonym Gene Names
BCR1; D22S11
NCBI Protein Information
breakpoint cluster region protein

NCBI Summary for BCR

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for BCR

GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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