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CDC25A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225208

Rabbit anti Cdc25A (pSer76) antibody recognizes M-phase inducer phosphatase 1 also known as dual specificity phosphatase Cdc25A, when phosphorylated at serine 76.Cdc25A belongs to the MPI phosphatase family and is involved in cell cycle progression.… More details

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Product Overview

Product Name
CDC25A, Polyclonal Antibody
Full Product Name
Rabbit anti CDC25A (pSer76)
Product Gene Name
Product Synonym Names
CDC25A (pSer76) Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
54,551 Da
OMIM
116947
3D Structure
ModBase 3D Structure for P30304
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 76 of human Cdc25A
Preperation
Antiserum to Cdc25A (pSer76) was raised by repeated immunization of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-CDC25A antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-CDC25A antibody
IHC-Paraffin: 1:50-1:100
WB: 1:500-1:2000

Western Blot (WB) of anti-CDC25A antibody

Western blot analysis of extracts from HeLa cells untreated or treated with TNFa + Calyculin A using Rabbit anti Cdc25A (pSer76) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with TNF-a (20ng/ml) and Calyculin A (100nM) for 30 minutes
Rabbit Anti-CDC25A Polyclonal Antibody – Cat# MBS225208

Immunohistochemistry (IHC) of anti-CDC25A antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti Cdc25A (pSer76) antibody
Rabbit Anti-CDC25A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS225208

Other Notes

Small volumes of anti-CDC25A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC25A purchase

MBS225208 is an antibody targeting CDC25A. Also known as Rabbit anti CDC25A (pSer76). Reactive in Human, Mouse (Expected from Sequence), Rat (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC25A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
M-phase inducer phosphatase 1
NCBI Official Synonym Full Names
cell division cycle 25A
NCBI Official Symbol
CDC25A
NCBI Official Synonym Symbols
CDC25A2
UniProt Protein Name
M-phase inducer phosphatase 1
UniProt Synonym Protein Names
Dual specificity phosphatase Cdc25A
UniProt Gene Name
CDC25A
NCBI Protein Information
M-phase inducer phosphatase 1

NCBI Summary for CDC25A

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CDC25A

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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