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EIF4E Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225234

Rabbit anti EIF4E (pSer209) antibody recognizes the eukaryotic translation initiation factor 4E (EIF4E), also known as eIF-4F 25 kDa subunit or mRNA cap-binding protein, when phosphorylated at serine 209. EIF4E is a member of the eukaryotic initiation factor… More details

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Product Overview

Product Name
EIF4E, Polyclonal Antibody
Full Product Name
Rabbit anti EIF4E (pSer209)
Product Gene Name
Product Synonym Names
EIF4E (pSer209) Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
27,260 Da
OMIM
133440
3D Structure
ModBase 3D Structure for P06730
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 209 of human EIF4E
Preperation
Antiserum to EIF4E (pSer209) was raised by repeated immunization of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-EIF4E antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-EIF4E antibody
IHC-Paraffin: 1:50-1:100
WB: 1:500-1:2000

Western Blot (WB) of anti-EIF4E antibody

Western blot analysis of extracts from SK-BR-3 cells untreated, treated with Insulin and EGF or treated with Insulin EGF and U0126 using Rabbit anti EIF4E (pSer209) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml for 30 minutes) and Insulin (100nM for 10 minutes) or treatment with U0126.
Rabbit Anti-EIF4E Polyclonal Antibody – Cat# MBS225234

Immunohistochemistry (IHC) of anti-EIF4E antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti EIF4E (pSer209) antibody
Rabbit Anti-EIF4E Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS225234

Other Notes

Small volumes of anti-EIF4E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4E purchase

MBS225234 is an antibody targeting EIF4E. Also known as Rabbit anti EIF4E (pSer209). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4E. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Eukaryotic translation initiation factor 4E
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E
NCBI Official Symbol
EIF4E
NCBI Official Synonym Symbols
CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
UniProt Protein Name
Eukaryotic translation initiation factor 4E
UniProt Synonym Protein Names
eIF-4F 25 kDa subunit; mRNA cap-binding protein
UniProt Gene Name
EIF4E
UniProt Synonym Gene Names
EIF4EL1; EIF4F; eIF-4E; eIF4E
NCBI Protein Information
eukaryotic translation initiation factor 4E

NCBI Summary for EIF4E

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
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UniProt Comments for EIF4E

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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