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Ezrin (EZR) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225249

Rabbit anti ezrin (pTyr353) antibody recognizes ezrin, also known as villin-2, when phosphorylated at tyrosine 353. Ezrin is a member of the Ezrin Radixin Mosin (ERM) family. Ezrin functions as a linker molecule between the plasma membrane and the… More details

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Product Overview

Product Name
Ezrin, Polyclonal Antibody
Full Product Name
Rabbit anti Ezrin (pTyr353)
Product Gene Name
Product Synonym Names
Ezrin (pTyr353) Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse (Expected from Sequence)
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
69,413 Da
OMIM
123900
3D Structure
ModBase 3D Structure for P15311
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 353 of human ezrin
Preperation
Antiserum to ezrin (pTyr353) was raised by repeated immunization of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-EZR antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-EZR antibody
IHC-Paraffin: 1:50-1:100
WB: 1:500-1:2000

Western Blot (WB) of anti-EZR antibody

Western blot analysis of extracts from HEK-293 cells untreated or treated with EGF using Rabbit anti ezrin (pTyr353) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes
Rabbit Anti-EZR Polyclonal Antibody – Cat# MBS225249

Immunohistochemistry (IHC) of anti-EZR antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ezrin (pTyr353) antibody
Rabbit Anti-EZR Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS225249

Other Notes

Small volumes of anti-EZR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EZR purchase

MBS225249 is an antibody targeting EZR. Also known as Rabbit anti Ezrin (pTyr353). Reactive in Human, Mouse (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EZR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Ezrin
NCBI Official Synonym Full Names
ezrin
NCBI Official Symbol
EZR
NCBI Official Synonym Symbols
CVL; CVIL; VIL2; HEL-S-105
UniProt Protein Name
Ezrin
UniProt Synonym Protein Names
Cytovillin; Villin-2; p81
Protein Family
UniProt Gene Name
EZR
UniProt Synonym Gene Names
VIL2
NCBI Protein Information
ezrin

NCBI Summary for EZR

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for EZR

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EZR antibody

 Reference Product  PubMed Publications
 PRKCA antibody  >1 publications with EZR and PRKCA
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