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FOXO3A (FOXO3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225255

Rabbit anti FOXO3A (pSer253) antibody recognizes FOXO3A, when phosphorylated at serine 253. FOXO3A is a member of the forkhead family of transcription factors. Residue 253 of FOXO3A is phosphorylated by a number of kinases including AKT. Phosphorylation of… More details

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Testing Data View PDF
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Product Overview

Product Name
FOXO3A, Polyclonal Antibody
Full Product Name
Rabbit anti FOXO3A (pSer253)
Product Gene Name
Product Synonym Names
FOXO3A (pSer253) Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
48,406 Da
OMIM
602681
3D Structure
ModBase 3D Structure for O43524
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 253 of human FOXO3A
Preperation
Antiserum to FOXO3A (pSer253) was raised by repeated immunization of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-FOXO3 antibody
Western Blot (WB)
Application Notes for anti-FOXO3 antibody
WB: 1:500-1:1000

Western Blot (WB) of anti-FOXO3 antibody

Western blot analysis of extracts from HEK-293 cells untreated or treated with EGF using Rabbit anti FOXO3A (pSer253) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes
Rabbit Anti-FOXO3 Polyclonal Antibody – Cat# MBS225255

Other Notes

Small volumes of anti-FOXO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FOXO3 purchase

MBS225255 is an antibody targeting FOXO3. Also known as Rabbit anti FOXO3A (pSer253). Reactive in Human, Mouse (Expected from Sequence), Rat (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FOXO3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Forkhead box protein O3
NCBI Official Synonym Full Names
forkhead box O3
NCBI Official Symbol
FOXO3
NCBI Official Synonym Symbols
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
UniProt Protein Name
Forkhead box protein O3
UniProt Synonym Protein Names
AF6q21 protein
Protein Family
UniProt Gene Name
FOXO3
NCBI Protein Information
forkhead box protein O3

NCBI Summary for FOXO3

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
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UniProt Comments for FOXO3

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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