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P27/Kip1 (CDKN1B) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225298

Rabbit anti p27/Kip1 (pThr187) antibody recognizes p27/Kip1, also known as cyclin-dependent kinase inhibitor p27 or cyclin-dependent kinase inhibitor 1B, when phosphorylated at threonine 187. p27/Kip1 is a cyclin-dependent kinase inhibitor (CKI) that belongs… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
p27/Kip1 (CDKN1B), Polyclonal Antibody
Full Product Name
Rabbit anti p27/Kip1 (pThr187)
Product Gene Name
Product Synonym Names
p27/Kip1 (pThr187) Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified whole serum by affinity chromatography.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
22,073 Da
OMIM
600778
3D Structure
ModBase 3D Structure for P46527
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 187 of human p27/Kip1
Preperation
Antiserum to p27/Kip1 (pThr187) was raised by repeated immunization of rabbits with highly purified antigen.
Applications Tested/Suitable for anti-CDKN1B antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-CDKN1B antibody
IHC-Paraffin: 1:50-1:100
WB: 1:500-1:1000

Western Blot (WB) of anti-CDKN1B antibody

Western blot analysis of extracts from HeLa cells untreated or treated with EGF using Rabbit anti p27/Kip1 (pThr187) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes
Rabbit Anti-CDKN1B Polyclonal Antibody – Cat# MBS225298

Immunohistochemistry (IHC) of anti-CDKN1B antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti p27/Kip1 (pThr187) antibody
Rabbit Anti-CDKN1B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS225298

Other Notes

Small volumes of anti-CDKN1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDKN1B purchase

MBS225298 is an antibody targeting CDKN1B. Also known as Rabbit anti p27/Kip1 (pThr187). Reactive in Human, Mouse (Expected from Sequence), Rat (Expected from Sequence). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDKN1B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Cyclin-dependent kinase inhibitor 1B
NCBI Official Synonym Full Names
cyclin dependent kinase inhibitor 1B
NCBI Official Symbol
CDKN1B
NCBI Official Synonym Symbols
KIP1; MEN4; CDKN4; MEN1B; P27KIP1
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1B
UniProt Synonym Protein Names
Cyclin-dependent kinase inhibitor p27
UniProt Gene Name
CDKN1B
UniProt Synonym Gene Names
KIP1
NCBI Protein Information
cyclin-dependent kinase inhibitor 1B

NCBI Summary for CDKN1B

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
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UniProt Comments for CDKN1B

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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