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TOLLIP Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225373

Rat anti TOLLIP antibody clone Kimmy-1 recognizes Toll-interacting protein (TOLLIP), an ubiquitin binding protein that plays an important role in regulating the innate immune response.TOLLIP, as the name implies, interacts with a number of Toll-like receptors… More details

$635 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TOLLIP, Monoclonal Antibody
Full Product Name
Rat anti TOLLIP
Product Gene Name
Product Synonym Names
TOLLIP Antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
Kimmy-1
Host
Rat
Species Reactivity
Mouse, Human
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide (NaN3).
Purity/Purification
Purified
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
30,345 Da
3D Structure
ModBase 3D Structure for Q9QZ06
Immunogen
Recombinant mouse TOLLIP
Applications Tested/Suitable for anti-Tollip antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-Tollip antibody
WB: 5 ug/ml

Testing Data of anti-Tollip antibody

25 ug murine EL4 whole cell lysate (lanes 2, and 3) was loaded onto a 4-15% Criterion„¢ TGX-Stain-Free„¢ Gel and transferred onto a nitrocellulose membrane. The membrane was blocked with Block ACE (BUF029) and probed with rat anti TOLLIP antibody at 5ug/ml in Block ACE wash (Lane 2) for 1 hour at room temperature. The membrane was washed 3x for 10 minutes with 5 ml Block ACE wash before addition of the HRP-conjugated goat anti-Rat IgG secondary antibody at 1/10,000 (Lanes 1-3). The membrane was washed 4x for 5 minutes with Block ACE wash and 2x in 5 ml PBS before developing with Clarity ECL on the Chemidoc. Lane 3 is a secondary only antibody control
Rat Anti-TOLLIP Monoclonal Antibody – Cat# MBS225373

Other Notes

Small volumes of anti-Tollip antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Tollip purchase

MBS225373 is an antibody targeting Tollip. Also known as Rat anti TOLLIP. Reactive in Mouse, Human. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Tollip. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Toll-interacting protein
NCBI Official Synonym Full Names
toll interacting protein
NCBI Official Symbol
Tollip
NCBI Official Synonym Symbols
4930403G24Rik; 4931428G15Rik
UniProt Protein Name
Toll-interacting protein
UniProt Gene Name
Tollip
NCBI Protein Information
toll-interacting protein

UniProt Comments for Tollip

Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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