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GATA3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225375

Mouse anti GATA3 antibody clone 1A12-1D9 recognizes GATA3 also known as trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3.The GATA3 protein is a member of the GATA family of transcription factors which has received its name… More details

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Product Documentation
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GATA3, Monoclonal Antibody
Full Product Name
Mouse anti GATA3
Product Gene Name
anti-GATA3 antibody
Product Synonym Names
GATA3 Antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1A12-1D9
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in phosphate buffered saline with 0.05% sodium azide (NaN3), 1 mg/ml bovine serum albumin, 30% glycerol
Purity/Purification
Purified by affinity chromatography on Protein A
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
48,045 Da
OMIM
131320
3D Structure
ModBase 3D Structure for P23771
Immunogen
Purified partial recombinant recombinant human GATA3 protein expressed in E Coli
Applications Tested/Suitable for anti-GATA3 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-GATA3 antibody
ICC: 1:50-1:100
IF: 1:50-1:100
IP: 5 ug
WB: 1:1000
IHC-Paraffin: 1:200-1:2000

Western Blot (WB) of anti-GATA3 antibody

Western blot analysis of GATA3 was performed by loading 15ug of SH-SY5Y whole cell lysate (WCL) and SH-SY5Y nuclear extract (NE) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked for at least 1 hour. The membrane was probed with Mouse anti GATA3 at a dilution of 1/1000 overnight at 4 degree C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody for 1 hour. Chemiluminescent detection was performed
Mouse Anti-GATA3 Monoclonal Antibody – Cat# MBS225375

Immunofluorescence (IF) of anti-GATA3 antibody

Immunofluorescent analysis of GATA3 (green) in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) Mouse anti GATA3 at a dilution of 1/50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody for 30 minutes at room temperature. F-Actin (red) was stained with phalloidin (red) and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20x magnification
Mouse Anti-GATA3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS225375

Immunohistochemistry (IHC) of anti-GATA3 antibody

Immunohistochemistry analysis of GATA3 showing staining in the nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with dH2O and PBS, and then probed with a Mouse anti GATA3 diluted in 3% BSA-PBS at a dilution of 1/1000 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting
Mouse Anti-GATA3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS225375

Other Notes

Small volumes of anti-GATA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GATA3 purchase

MBS225375 is an antibody targeting GATA3. Also known as Mouse anti GATA3. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GATA3. It may not necessarily be applicable to this product.

NCBI GI #
120962
NCBI GeneID
2625
NCBI Accession #
P23771.1
UniProt Primary Accession #
P23771
UniProt Secondary Accession #
Q5VWG7; Q5VWG8; Q96J16
UniProt Related Accession #
P23771
NCBI Official Full Name
Trans-acting T-cell-specific transcription factor GATA-3
NCBI Official Synonym Full Names
GATA binding protein 3
NCBI Official Symbol
GATA3
NCBI Official Synonym Symbols
HDR; HDRS
UniProt Protein Name
Trans-acting T-cell-specific transcription factor GATA-3
UniProt Synonym Protein Names
GATA-binding factor 3
Protein Family
GATA-binding factor
UniProt Gene Name
GATA3
NCBI Protein Information
trans-acting T-cell-specific transcription factor GATA-3

NCBI Summary for GATA3

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
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UniProt Comments for GATA3

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.

Research Articles on GATA3

1. GATA3 interacts with and is acetylated by the acetyltransferase CBP. The major acetylated site of GATA3 in lung adenocarcinoma cells is lysine 119.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GATA3 antibody

 Reference Product  PubMed Publications
 TBX21 antibody  >295 publications with GATA3 and TBX21
 IL13 antibody  >96 publications with GATA3 and IL13
 IL10 antibody  >78 publications with GATA3 and IL10
 IL4 antibody  >73 publications with GATA3 and IL4
 NOTCH1 antibody  >30 publications with GATA3 and NOTCH1
 IL5 antibody  >22 publications with GATA3 and IL5
 STAT1 antibody  >20 publications with GATA3 and STAT1
 MYB antibody  >14 publications with GATA3 and MYB
 ZFPM2 antibody  >4 publications with GATA3 and ZFPM2
 EP300 antibody  >3 publications with GATA3 and EP300

Pathways associated with anti-GATA3 antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL27-mediated Signaling Events Pathway antibodies  IL27-mediated Signaling Events Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram

Diseases associated with anti-GATA3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >246 publications with GATA3 and Inflammation
 Lung Diseases Antibodies  >184 publications with GATA3 and Lung Diseases
 Disease Models, Animal Antibodies  >126 publications with GATA3 and Disease Models, Animal
 Breast Neoplasms Antibodies  >121 publications with GATA3 and Breast Neoplasms
 Carcinoma Antibodies  >121 publications with GATA3 and Carcinoma
 Kidney Diseases Antibodies  >65 publications with GATA3 and Kidney Diseases
 Adenocarcinoma Antibodies  >59 publications with GATA3 and Adenocarcinoma
 Necrosis Antibodies  >56 publications with GATA3 and Necrosis
 Cardiovascular Diseases Antibodies  >41 publications with GATA3 and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >26 publications with GATA3 and Lung Neoplasms

Organs/Tissues associated with anti-GATA3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1062 publications with GATA3 and Blood
 Lung Antibodies  >164 publications with GATA3 and Lung
 Kidney Antibodies  >119 publications with GATA3 and Kidney
 Embryonic Tissue Antibodies  >83 publications with GATA3 and Embryonic Tissue
 Thymus Antibodies  >78 publications with GATA3 and Thymus
 Brain Antibodies  >73 publications with GATA3 and Brain
 Skin Antibodies  >70 publications with GATA3 and Skin
 Lymph Node Antibodies  >70 publications with GATA3 and Lymph Node
 Liver Antibodies  >64 publications with GATA3 and Liver
 Ear Antibodies  >55 publications with GATA3 and Ear
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