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Vesicle-Fusing Atpase (NSF) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225377

Mouse anti vesicle-fusing ATPase antibody, clone 9G7-3 recognizes vesicle-fusing ATPase also known as NEM-sensitive fusion protein or NSF.Vesicle-fusing ATPase is a member of the hydrolase protein family, which play an essential role in vesicle-mediated… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
Vesicle-fusing ATPase (NSF), Monoclonal Antibody
Full Product Name
Mouse anti Vesicle-fusing ATPase
Product Gene Name
Product Synonym Names
Vesicle-fusing ATPase Antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9G7-3
Host
Mouse
Species Reactivity
Human, Rat, Bovine, Hamster, Monkey, Pig, Mouse, Rabbit, Sheep
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3), 50% glycerol.
Purity/Purification
Purified by affinity chromatography on Protein G
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Molecular Weight
71,584 Da
OMIM
601633
3D Structure
ModBase 3D Structure for P46459
Immunogen
Recombinant bovine vesicle-fusing ATPase
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the murine NS-1 myeloma.
Applications Tested/Suitable for anti-NSF antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-NSF antibody
WB: 1:1000

Western Blot (WB) of anti-NSF antibody

Western blot analysis of HeLa (heat shocked; lane 2), Vero (lane 3), CHO-K1 (lane 4) and ESK-4 (lane 5) cell lysates probed with Mouse anti vesicle-fusing ATPase . Molecular weight marker is shown in lane 1
Mouse Anti-NSF Monoclonal Antibody – Cat# MBS225377

Other Notes

Small volumes of anti-NSF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NSF purchase

MBS225377 is an antibody targeting NSF. Also known as Mouse anti Vesicle-fusing ATPase. Reactive in Human, Rat, Bovine, Hamster, Monkey, Pig, Mouse, Rabbit, Sheep. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NSF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Vesicle-fusing ATPase
NCBI Official Synonym Full Names
N-ethylmaleimide sensitive factor, vesicle fusing ATPase
NCBI Official Symbol
NSF
NCBI Official Synonym Symbols
SKD2; SEC18
UniProt Protein Name
Vesicle-fusing ATPase
UniProt Synonym Protein Names
N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF
Protein Family
UniProt Gene Name
NSF
UniProt Synonym Gene Names
NEM-sensitive fusion protein
NCBI Protein Information
vesicle-fusing ATPase

UniProt Comments for NSF

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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