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PTPN11 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225446

MBS225446 is an antibody targeting PTPN11. Also known as Mouse anti PTPN11/6. Reactive in Human, Mouse, Rat. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PTPN11/6, Monoclonal Antibody
Full Product Name
Mouse anti PTPN11/6
Product Gene Name
Product Synonym Names
PTPN11/6 Antibody
Clonality
Monoclonal
Isotype
IgG2b
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Store undiluted at -20 degree C. Avoid repeated freeze/thaw cycles. Shelf Life: As supplied, 12 months from date of despatch.
Molecular Weight
68 kDa
OMIM
151100
3D Structure
ModBase 3D Structure for Q06124
Immunogen
E Coli derived recombinant protein of human PTPN11
Applications Tested/Suitable for anti-PTPN11 antibody
Western Blot (WB)
Application Notes for anti-PTPN11 antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 68 kDa in Jurkat cell lysates.

Western Blot (WB) of anti-PTPN11 antibody

Western blot analysis of whole cell lysates probed with PTPN11/6 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to PTPN11 and PTPN6 (molecular weight 68 kDa)
Mouse Anti-PTPN11 Monoclonal Antibody – Cat# MBS225446

Western Blot (WB) of anti-PTPN11 antibody

Western blot analysis of whole cell lysates probed with PTPN11/6 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to PTPN11 and PTPN6 (molecular weight 68 kDa)
Mouse Anti-PTPN11 Monoclonal Antibody, Western Blot (WB) – Cat# MBS225446

Other Notes

Small volumes of anti-PTPN11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PTPN11. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Tyrosine-protein phosphatase non-receptor type 11
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 11
NCBI Official Symbol
PTPN11
NCBI Official Synonym Symbols
CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 11
UniProt Synonym Protein Names
Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3
UniProt Gene Name
PTPN11
UniProt Synonym Gene Names
PTP2C; SHPTP2; PTP-1D; PTP-2C; SHP-2; Shp2
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 11

NCBI Summary for PTPN11

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]
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UniProt Comments for PTPN11

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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