Mouse Anti-ABCC1 Monoclonal Antibody – Cat# MBS225455

Western blot analysis of whole cell lysates probed with ABCC1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 41.5 second exposure. Arrow points to ABCC1 (molecular weight 172 kDa)

ABCC1 Antibody – Mouse Monoclonal

Name: ABCC1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS225455
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225455

MBS225455 is an antibody targeting ABCC1. Also known as ABCC1 Antibody with Control Lysate. Reactive in Human. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ABCC1, Monoclonal Antibody

Full Product Name

ABCC1 Antibody with Control Lysate

Product Gene Name

anti-ABCC1 antibody

Product Synonym Names

Mouse anti ABCC1; ABCC1 Antibody

Clonality

Monoclonal

Isotype

IgG1

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).

Purity/Purification

Purified by affinity chromatography on Protein G from tissue culture supernatant

Preparation and Storage

Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.

Molecular Weight

172 kDa

OMIM

158343

3D Structure

ModBase 3D Structure for P33527

Immunogen

Recombinant protein corresponding to amino acids 194-316 of human ABCC1 expressed in E Coli

Lysate Composition

400ug HepG2 lysate lyophilized in RIPA buffer.

Lysate Reconstitution

If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u

Applications Tested/Suitable for anti-ABCC1 antibody

Western Blot (WB)

Application Notes for anti-ABCC1 antibody

WB: 1:1000
Western Blotting: Detects a band of approximately 172 kDa in HepG2 cell lysates.

Other Notes

Small volumes of anti-ABCC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ABCC1. It may not necessarily be applicable to this product.

NCBI GI #

296439301

NCBI GeneID

4363

NCBI Accession #

P33527.3

UniProt Primary Accession #

P33527

UniProt Secondary Accession #

O14819; O43333; P78419; Q59GI9; Q9UQ97; Q9UQ99; Q9UQA0; A3RJX2; C9JPJ4

UniProt Related Accession #

P33527

NCBI Official Full Name

Multidrug resistance-associated protein 1

NCBI Official Synonym Full Names

ATP binding cassette subfamily C member 1

NCBI Official Symbol

ABCC1

NCBI Official Synonym Symbols

MRP; ABCC; GS-X; MRP1; ABC29

UniProt Protein Name

Multidrug resistance-associated protein 1

UniProt Synonym Protein Names

ATP-binding cassette sub-family C member 1; Leukotriene C(4) transporter; LTC4 transporter

Protein Family

ABC transporter C family

UniProt Gene Name

ABCC1

UniProt Synonym Gene Names

MRP; MRP1; LTC4 transporter

NCBI Protein Information

multidrug resistance-associated protein 1

NCBI Summary for ABCC1

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown. [provided by RefSeq, Apr 2012]

UniProt Comments for ABCC1

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.

Research Articles on ABCC1

1. No significant correlation was observed between rs504348 genotypes and cystic fibrosis severity. A significantly higher expression of ABCC1 mRNA was induced by CF plasma compared to healthy control plasma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ABCC1 antibody

Products by Pathway	Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC-family Proteins Mediated Transport Pathway antibodies	ABC-family Proteins Mediated Transport Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway antibodies	Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway Diagram
Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective BTD Causes Biotidinase Deficiency Pathway antibodies	Defective BTD Causes Biotidinase Deficiency Pathway Diagram
Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies	Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies	Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram

Diseases associated with anti-ABCC1 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>47 publications with ABCC1 and Breast Neoplasms
Lung Neoplasms Antibodies	>29 publications with ABCC1 and Lung Neoplasms
Brain Diseases Antibodies	>28 publications with ABCC1 and Brain Diseases
Nervous System Diseases Antibodies	>28 publications with ABCC1 and Nervous System Diseases
Adenocarcinoma Antibodies	>27 publications with ABCC1 and Adenocarcinoma
Liver Diseases Antibodies	>23 publications with ABCC1 and Liver Diseases
Disease Models, Animal Antibodies	>20 publications with ABCC1 and Disease Models, Animal
Ovarian Neoplasms Antibodies	>16 publications with ABCC1 and Ovarian Neoplasms
Liver Neoplasms Antibodies	>16 publications with ABCC1 and Liver Neoplasms
Colorectal Neoplasms Antibodies	>13 publications with ABCC1 and Colorectal Neoplasms

Organs/Tissues associated with anti-ABCC1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>129 publications with ABCC1 and Blood
Brain Antibodies	>74 publications with ABCC1 and Brain
Liver Antibodies	>66 publications with ABCC1 and Liver
Lung Antibodies	>57 publications with ABCC1 and Lung
Kidney Antibodies	>46 publications with ABCC1 and Kidney
Vascular Antibodies	>23 publications with ABCC1 and Vascular
Intestine Antibodies	>23 publications with ABCC1 and Intestine
Testis Antibodies	>15 publications with ABCC1 and Testis
Skin Antibodies	>13 publications with ABCC1 and Skin
Placenta Antibodies	>12 publications with ABCC1 and Placenta