• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Clathrin Heavy Chain (CLTC) Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225481

MBS225481 is an antibody targeting CLTC. Also known as Clathrin Heavy Chain Antibody with Control Lysate. Reactive in Human, Mouse, Rat. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
Clathrin Heavy Chain (CLTC), Monoclonal Antibody
Full Product Name
Clathrin Heavy Chain Antibody with Control Lysate
Product Gene Name
Product Synonym Names
Mouse anti CLATHRIN HEAVY CHAIN; Clathrin Heavy Chain Antibody
Clonality
Monoclonal
Isotype
IgG1
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch
Molecular Weight
192 kDa
OMIM
118955
3D Structure
ModBase 3D Structure for Q00610
Immunogen
E Coli-derived recombinant protein corresponding to aa 1521-1675 of human clathrin heavy chain
Lysate Composition
400ug HepG2 lysate lyophilized in RIPA buffer
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Applications Tested/Suitable for anti-CLTC antibody
Western Blot (WB)
Application Notes for anti-CLTC antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 192 kDa in HepG2 cell lysates.

Western Blot (WB) of anti-CLTC antibody

Western blot analysis of whole cell lysates probed with clathrin heavy chain antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemicDoc„¢ MP with 4 second exposure. Arrow points to clathrin heavy chain (molecular weight 192 kDa)
Anti-CLTC Monoclonal Antibody – Cat# MBS225481

Other Notes

Small volumes of anti-CLTC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CLTC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Clathrin heavy chain 1
NCBI Official Synonym Full Names
clathrin heavy chain
NCBI Official Symbol
CLTC
NCBI Official Synonym Symbols
Hc; CHC; CHC17; MRD56; CLH-17; CLTCL2
UniProt Protein Name
Clathrin heavy chain 1
UniProt Synonym Protein Names
Clathrin heavy chain on chromosome 17; CLH-17
Protein Family
UniProt Gene Name
CLTC
UniProt Synonym Gene Names
CLH17; CLTCL2; KIAA0034; CLH-17
NCBI Protein Information
clathrin heavy chain 1

NCBI Summary for CLTC

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for CLTC

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872).
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000