Mouse Anti-HMGA2 Monoclonal Antibody – Cat# MBS225487

Western blot analysis of whole cell lysates probed with high mobility group protein HMGI-C antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemicDoc„¢ MP with 56 second exposure. Arrow points to high mobility group protein HMGI-C (molecular weight 18 kDa)

High Mobility Group Protein HMGI-C (HMGA2) Antibody – Mouse Monoclonal

Name: HMGA2 Antibody; Monoclonal; Mouse; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS225487
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225487

MBS225487 is an antibody targeting HMGA2. Also known as High Mobility Group Protein HMGI-C Antibody with Control Lysate. Reactive in Human, Mouse. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

High Mobility Group Protein HMGI-C (HMGA2), Monoclonal Antibody

Full Product Name

High Mobility Group Protein HMGI-C Antibody with Control Lysate

Product Gene Name

anti-HMGA2 antibody

Product Synonym Names

Mouse anti HIGH MOBILITY GROUP PROTEIN HMGI-C; High Mobility Group Protein HMGI-C Antibody

Clonality

Monoclonal

Isotype

IgG1

Host

Mouse

Species Reactivity

Human, Mouse

Form/Format

Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).

Purity/Purification

Purified by affinity chromatography on Protein G from tissue culture supernatant

Preparation and Storage

Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch

Molecular Weight

18 kDa

OMIM

150699

3D Structure

ModBase 3D Structure for P52926

Immunogen

E Coli-derived recombinant protein corresponding to aa 1-109 of human high mobility group protein HMGI-C

Lysate Composition

400ug HepG2 lysate lyophilized in RIPA buffer

Lysate Reconstitution

If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u

Applications Tested/Suitable for anti-HMGA2 antibody

Western Blot (WB)

Application Notes for anti-HMGA2 antibody

WB: 1:1000
Western Blotting: Detects a band of approximately 18 kDa in HepG2 cell lysates.

Other Notes

Small volumes of anti-HMGA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HMGA2. It may not necessarily be applicable to this product.

NCBI GI #

1708263

NCBI GeneID

8091

NCBI Accession #

P52926.1

UniProt Primary Accession #

P52926

UniProt Secondary Accession #

Q1M182; Q1M185; Q1M186; Q1M187; Q1M188; E7EP85; E7EWA2

UniProt Related Accession #

P52926

NCBI Official Full Name

High mobility group protein HMGI-C

NCBI Official Synonym Full Names

high mobility group AT-hook 2

NCBI Official Symbol

HMGA2

NCBI Official Synonym Symbols

BABL; LIPO; HMGIC; HMGI-C; STQTL9

UniProt Protein Name

High mobility group protein HMGI-C

UniProt Synonym Protein Names

High mobility group AT-hook protein 2

Protein Family

High mobility group protein

UniProt Gene Name

HMGA2

UniProt Synonym Gene Names

HMGIC

NCBI Protein Information

high mobility group protein HMGI-C

NCBI Summary for HMGA2

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

UniProt Comments for HMGA2

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation ().

Research Articles on HMGA2

1. This study demonstrated that the transactivation of FOXL2 driven by interactions between HMGA2 and pRb might exert critical effects on the metastases and EMT of chemoresistant gastric cancer. Blocking the HMGA2-FOXL2-ITGA2 pathway could serve as a new strategy for gastric cancer treatment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HMGA2 antibody

Reference Product	PubMed Publications
LPP antibody	>28 publications with HMGA2 and LPP
CDH1 antibody	>5 publications with HMGA2 and CDH1
SMAD3 antibody	>5 publications with HMGA2 and SMAD3
IGF2BP1 antibody	>2 publications with HMGA2 and IGF2BP1
COX6C antibody	>2 publications with HMGA2 and COX6C
IGF2BP3 antibody	>2 publications with HMGA2 and IGF2BP3
BMP4 antibody	>2 publications with HMGA2 and BMP4
SMAD2 antibody	>2 publications with HMGA2 and SMAD2
SMAD4 antibody	>1 publications with HMGA2 and SMAD4

Pathways associated with anti-HMGA2 antibody

Products by Pathway	Pathway Diagram
Cellular Senescence Pathway antibodies	Cellular Senescence Pathway Diagram
Cellular Responses To Stress Pathway antibodies	Cellular Responses To Stress Pathway Diagram
DNA Damage/Telomere Stress Induced Senescence Pathway antibodies	DNA Damage/Telomere Stress Induced Senescence Pathway Diagram
Formation Of Senescence-Associated Heterochromatin Foci (SAHF) Pathway antibodies	Formation Of Senescence-Associated Heterochromatin Foci (SAHF) Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-HMGA2 antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>122 publications with HMGA2 and Carcinoma
LIPOMATOSIS, MULTIPLE Antibodies	>94 publications with HMGA2 and LIPOMATOSIS, MULTIPLE
Cell Transformation, Neoplastic Antibodies	>69 publications with HMGA2 and Cell Transformation, Neoplastic
Adenocarcinoma Antibodies	>66 publications with HMGA2 and Adenocarcinoma
Lung Neoplasms Antibodies	>40 publications with HMGA2 and Lung Neoplasms
Nervous System Diseases Antibodies	>32 publications with HMGA2 and Nervous System Diseases
Hematologic Diseases Antibodies	>32 publications with HMGA2 and Hematologic Diseases
Breast Neoplasms Antibodies	>30 publications with HMGA2 and Breast Neoplasms
Skin Diseases Antibodies	>30 publications with HMGA2 and Skin Diseases
Neoplasms, Experimental Antibodies	>30 publications with HMGA2 and Neoplasms, Experimental

Organs/Tissues associated with anti-HMGA2 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>60 publications with HMGA2 and Lung
Embryonic Tissue Antibodies	>46 publications with HMGA2 and Embryonic Tissue
Thyroid Antibodies	>36 publications with HMGA2 and Thyroid
Bone Antibodies	>34 publications with HMGA2 and Bone
Uterus Antibodies	>25 publications with HMGA2 and Uterus
Mouth Antibodies	>21 publications with HMGA2 and Mouth
Connective Tissue Antibodies	>21 publications with HMGA2 and Connective Tissue
Vascular Antibodies	>19 publications with HMGA2 and Vascular
Liver Antibodies	>17 publications with HMGA2 and Liver
Heart Antibodies	>16 publications with HMGA2 and Heart