• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

C-Myc (MYC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225491

MBS225491 is an antibody targeting MYC. Also known as c-Myc Antibody with Control Lysate. Reactive in Human, Mouse, Rat. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
c-Myc, Monoclonal Antibody
Full Product Name
c-Myc Antibody with Control Lysate
Product Gene Name
Product Synonym Names
Mouse anti C-MYC; c-Myc Antibody
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Molecular Weight
57 kDa
OMIM
113970
3D Structure
ModBase 3D Structure for P01106
Immunogen
E Coli derived recombinant protein corresponding to amino acids 66-201 of human c-Myc
Lysate Composition
400ug HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Applications Tested/Suitable for anti-MYC antibody
Western Blot (WB)
Application Notes for anti-MYC antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 57 kDa in HeLa cell lysate.

Western Blot (WB) of anti-MYC antibody

Western blot analysis of whole cell lysates probed with c-Myc antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 41.5 second exposure. Arrow points to c-Myc (molecular weight 57 kDa)
Mouse Anti-MYC Monoclonal Antibody – Cat# MBS225491

Other Notes

Small volumes of anti-MYC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MYC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Myc proto-oncogene protein
NCBI Official Synonym Full Names
MYC proto-oncogene, bHLH transcription factor
NCBI Official Symbol
MYC
NCBI Official Synonym Symbols
MRTL; MYCC; c-Myc; bHLHe39
UniProt Protein Name
Myc proto-oncogene protein
UniProt Synonym Protein Names
Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64
Protein Family
UniProt Gene Name
MYC
UniProt Synonym Gene Names
BHLHE39; bHLHe39
NCBI Protein Information
myc proto-oncogene protein

NCBI Summary for MYC

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
Show more

UniProt Comments for MYC

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000