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EEF1A1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225497

MBS225497 is an antibody targeting EEF1A1. Also known as eEF1A1 Antibody with Control Lysate. Reactive in Human, Mouse, Rat. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
eEF1A1, Monoclonal Antibody
Full Product Name
eEF1A1 Antibody with Control Lysate
Product Gene Name
Product Synonym Names
Mouse anti eEF1A1; eEF1A1 Antibody
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Molecular Weight
50 kDa
OMIM
130590
3D Structure
ModBase 3D Structure for P68104
Immunogen
E Coli derived recombinant protein corresponding to amino acids 1-462 of human eEF1A1
Lysate Composition
400ug HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Applications Tested/Suitable for anti-EEF1A1 antibody
Western Blot (WB)
Application Notes for anti-EEF1A1 antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 50 kDa in HEK293 cell lysate

Western Blot (WB) of anti-EEF1A1 antibody

Western blot analysis of whole cell lysates probed with eEF1A1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 6 second exposure. Arrow points to eEF1A1 (molecular weight 50 kDa)
Mouse Anti-EEF1A1 Monoclonal Antibody – Cat# MBS225497

Other Notes

Small volumes of anti-EEF1A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EEF1A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Elongation factor 1-alpha 1
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 1
NCBI Official Symbol
EEF1A1
NCBI Official Synonym Symbols
CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
UniProt Protein Name
Elongation factor 1-alpha 1
UniProt Synonym Protein Names
Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7
UniProt Gene Name
EEF1A1
UniProt Synonym Gene Names
EEF1A; EF1A; LENG7; EF-1-alpha-1; EF-Tu; eEF1A-1
NCBI Protein Information
elongation factor 1-alpha 1

NCBI Summary for EEF1A1

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]
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UniProt Comments for EEF1A1

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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