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Spectrin Beta (SPTBN1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225500

MBS225500 is an antibody targeting SPTBN1. Also known as Mouse anti Spectrin Beta. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Spectrin Beta (SPTBN1), Monoclonal Antibody
Full Product Name
Mouse anti Spectrin Beta
Product Gene Name
Product Synonym Names
Spectrin Beta Antibody
Clonality
Monoclonal
Isotype
IgG2b
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Store undiluted at -20 degree C. Avoid repeated freeze/thaw cycles. Shelf Life: As supplied, 12 months from date of despatch.
Molecular Weight
275 kDa
OMIM
182790
3D Structure
ModBase 3D Structure for Q01082
Immunogen
E Coli derived recombinant protein corresponding to amino acids 2089-2364 of human spectrin beta
Applications Tested/Suitable for anti-SPTBN1 antibody
Western Blot (WB)
Application Notes for anti-SPTBN1 antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 275 kDa in Jurkat cell lysate

Western Blot (WB) of anti-SPTBN1 antibody

Western blot analysis of whole cell lysates probed with spectrin beta antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 31 second exposure. Arrow points to spectrin beta (molecular weight 275 kDa)
Mouse Anti-SPTBN1 Monoclonal Antibody – Cat# MBS225500

Other Notes

Small volumes of anti-SPTBN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPTBN1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Spectrin beta chain, non-erythrocytic 1
NCBI Official Synonym Full Names
spectrin beta, non-erythrocytic 1
NCBI Official Symbol
SPTBN1
NCBI Official Synonym Symbols
ELF; SPTB2; HEL102; betaSpII
UniProt Protein Name
Spectrin beta chain, non-erythrocytic 1
UniProt Synonym Protein Names
Beta-II spectrin; Fodrin beta chain; Spectrin, non-erythroid beta chain 1
Protein Family
UniProt Gene Name
SPTBN1
UniProt Synonym Gene Names
SPTB2
NCBI Protein Information
spectrin beta chain, non-erythrocytic 1

NCBI Summary for SPTBN1

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for SPTBN1

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPTBN1 antibody

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