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CLIC1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225525

MBS225525 is an antibody targeting CLIC1. Also known as CLIC1 Antibody with Control Lysate. Reactive in Human, Mouse, Rat. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CLIC1, Monoclonal Antibody
Full Product Name
CLIC1 Antibody with Control Lysate
Product Gene Name
Product Synonym Names
Mouse anti CLIC1; CLIC1 Antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
CPTC32
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Molecular Weight
27 kDa
OMIM
602872
3D Structure
ModBase 3D Structure for O00299
Immunogen
Recombinant protein of human CLIC1
Lysate Composition
400ug K562 lysate lyophilized in RIPA buffer
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Applications Tested/Suitable for anti-CLIC1 antibody
Western Blot (WB)
Application Notes for anti-CLIC1 antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 27 kDa in K562 cell lysates.

Western Blot (WB) of anti-CLIC1 antibody

Western blot analysis of whole cell lysates probed with CLIC1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 138 second exposure. Arrow points to CLIC1 (molecular weight 27 kDa)
Mouse Anti-CLIC1 Monoclonal Antibody – Cat# MBS225525

Other Notes

Small volumes of anti-CLIC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CLIC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Chloride intracellular channel protein 1
NCBI Official Synonym Full Names
chloride intracellular channel 1
NCBI Official Symbol
CLIC1
NCBI Official Synonym Symbols
G6; CL1C1; NCC27
UniProt Protein Name
Chloride intracellular channel protein 1
UniProt Synonym Protein Names
Chloride channel ABP; Nuclear chloride ion channel 27; NCC27; Regulatory nuclear chloride ion channel protein; hRNCC
UniProt Gene Name
CLIC1
UniProt Synonym Gene Names
G6; NCC27; NCC27; hRNCC
NCBI Protein Information
chloride intracellular channel protein 1

NCBI Summary for CLIC1

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008]
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UniProt Comments for CLIC1

Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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