Mouse Anti-CAND1 Monoclonal Antibody – Cat# MBS225536

Western blot analysis of whole cell lysates probed with CAND1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 16 second exposure. Arrow points to CAND1 (molecular weight 136 kDa)

CAND1 Antibody – Mouse Monoclonal

Name: CAND1 Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS225536
Price: 525 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225536

MBS225536 is an antibody targeting CAND1. Also known as CAND1 Antibody with Control Lysate. Reactive in Human, Mouse, Rat. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CAND1, Monoclonal Antibody

Full Product Name

CAND1 Antibody with Control Lysate

Product Gene Name

anti-CAND1 antibody

Product Synonym Names

Mouse anti CAND1; CAND1 Antibody

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

CD01/4H2

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).

Purity/Purification

Purified by affinity chromatography on Protein G from tissue culture supernatant

Preparation and Storage

Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.

Molecular Weight

136 kDa

OMIM

607727

3D Structure

ModBase 3D Structure for Q86VP6

Immunogen

E Coli-derived recombinant human CAND1 (amino acids 1 - 100)

Lysate Composition

400ug HEK293 lysate lyophilized in RIPA buffer

Lysate Reconstitution

If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u

Applications Tested/Suitable for anti-CAND1 antibody

Western Blot (WB)

Application Notes for anti-CAND1 antibody

WB: 1:1000
Western Blotting: Detects a band of approximately 136 kDa in HEK293 cell lysates.

Other Notes

Small volumes of anti-CAND1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CAND1. It may not necessarily be applicable to this product.

NCBI GI #

67460541

NCBI GeneID

55832

NCBI Accession #

Q86VP6.2

UniProt Primary Accession #

Q86VP6

UniProt Secondary Accession #

O94918; Q6PIY4; Q8NDJ4; Q96JZ9; Q96T19; Q9BTC4; Q9H0G2; Q9P0H7; Q9UF85; B2RAU3

UniProt Related Accession #

Q86VP6

NCBI Official Full Name

Cullin-associated NEDD8-dissociated protein 1

NCBI Official Synonym Full Names

cullin associated and neddylation dissociated 1

NCBI Official Symbol

CAND1

NCBI Official Synonym Symbols

TIP120; TIP120A

UniProt Protein Name

Cullin-associated NEDD8-dissociated protein 1

UniProt Synonym Protein Names

Cullin-associated and neddylation-dissociated protein 1; TBP-interacting protein of 120 kDa A; TBP-interacting protein 120A; p120 CAND1

Protein Family

Cullin-associated NEDD8-dissociated protein

UniProt Gene Name

CAND1

UniProt Synonym Gene Names

KIAA0829; TIP120; TIP120A; TBP-interacting protein 120A

NCBI Protein Information

cullin-associated NEDD8-dissociated protein 1

NCBI Summary for CAND1

This gene encodes an essential regulator of Cullin-RING ubiquitin ligases, which are in involved in ubiquitinylation of proteins degraded by the Ub proteasome system. The encoded protein binds to unneddylated cullin-RING box protein complexes and acts as an inhibitor of cullin neddylation and of Skp1, cullin, and F box ubiquitin ligase complex assembly and activity. In mammalian cell culture, this protein predominantly localizes to the cytoplasm. Knockdown of this gene in preadipocytes results in blocked adipogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]

UniProt Comments for CAND1

Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.

Research Articles on CAND1

1. describe the identification of the structural determinants responsible for the CA IX/CAND1 interaction

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CAND1 antibody

Reference Product	PubMed Publications
CUL1 antibody	>35 publications with CAND1 and CUL1
RBX1 antibody	>28 publications with CAND1 and RBX1
CUL3 antibody	>11 publications with CAND1 and CUL3
CUL2 antibody	>7 publications with CAND1 and CUL2
CUL4A antibody	>4 publications with CAND1 and CUL4A
CUL5 antibody	>3 publications with CAND1 and CUL5
CUL4B antibody	>3 publications with CAND1 and CUL4B

Pathways associated with anti-CAND1 antibody

Products by Pathway	Pathway Diagram
Endoderm Differentiation Pathway antibodies	Endoderm Differentiation Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Neutrophil Degranulation Pathway antibodies	Neutrophil Degranulation Pathway Diagram

Diseases associated with anti-CAND1 antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>4 publications with CAND1 and Kidney Diseases
Lung Neoplasms Antibodies	>3 publications with CAND1 and Lung Neoplasms
Lung Diseases Antibodies	>2 publications with CAND1 and Lung Diseases
Chromosome Aberrations Antibodies	>1 publications with CAND1 and Chromosome Aberrations
Liver Neoplasms Antibodies	>1 publications with CAND1 and Liver Neoplasms
Hypertension Antibodies	>1 publications with CAND1 and Hypertension
Cell Transformation, Neoplastic Antibodies	>1 publications with CAND1 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-CAND1 antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>6 publications with CAND1 and Vascular
Lung Antibodies	>5 publications with CAND1 and Lung
Liver Antibodies	>4 publications with CAND1 and Liver
Prostate Antibodies	>4 publications with CAND1 and Prostate
Blood Antibodies	>4 publications with CAND1 and Blood
Brain Antibodies	>4 publications with CAND1 and Brain
Bone Antibodies	>3 publications with CAND1 and Bone
Muscle Antibodies	>3 publications with CAND1 and Muscle
Heart Antibodies	>2 publications with CAND1 and Heart
Bone Marrow Antibodies	>2 publications with CAND1 and Bone Marrow