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EIF2A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225557

MBS225557 is an antibody targeting EIF2A. Also known as Mouse anti EIF2A. Reactive in Human, Mouse, Rat. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
EIF2A, Monoclonal Antibody
Full Product Name
Mouse anti EIF2A
Product Gene Name
Product Synonym Names
EIF2A Antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
CD02/2H5
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation and Storage
Store undiluted at -20 degree C. Avoid repeated freeze/thaw cycles. Shelf Life: As supplied, 12 months from date of despatch
Molecular Weight
62 kDa
OMIM
609234
3D Structure
ModBase 3D Structure for Q9BY44
Immunogen
E Coli-derived recombinant human EIF2A (aa 448-576)
Applications Tested/Suitable for anti-EIF2A antibody
Western Blot (WB)
Application Notes for anti-EIF2A antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 62 kDa in Jurkat cell lysates.

Western Blot (WB) of anti-EIF2A antibody

Western blot analysis of whole cell lysates probed with EIF2A antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 77 second exposure. Arrow points to EIF2A (molecular weight 62 kDa).
Mouse Anti-EIF2A Monoclonal Antibody – Cat# MBS225557

Other Notes

Small volumes of anti-EIF2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF2A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Eukaryotic translation initiation factor 2A
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2A
NCBI Official Symbol
EIF2A
NCBI Official Synonym Symbols
CDA02; EIF-2A; MST089; MSTP004; MSTP089
UniProt Protein Name
Eukaryotic translation initiation factor 2A
UniProt Synonym Protein Names
65 kDa eukaryotic translation initiation factor 2A
UniProt Gene Name
EIF2A
UniProt Synonym Gene Names
eIF-2A
NCBI Protein Information
eukaryotic translation initiation factor 2A

NCBI Summary for EIF2A

This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
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UniProt Comments for EIF2A

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF2A antibody

 Reference Product  PubMed Publications
 EIF2AK2 antibody  >1 publications with EIF2A and EIF2AK2
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