Mouse Anti-PRPF8 Monoclonal Antibody – Cat# MBS225559

Western blot analysis of whole cell lysates probed with PRPF8 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 118second exposure. Arrow points to PRPF8 (molecular weight 252 kDa).

PRPF8 Antibody – Mouse Monoclonal

Name: PRPF8 Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS225559
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225559

MBS225559 is an antibody targeting PRPF8. Also known as PRPF8 Antibody with Control Lysate. Reactive in Human, Mouse, Rat. Host: Mouse. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PRPF8, Monoclonal Antibody

Full Product Name

PRPF8 Antibody with Control Lysate

Product Gene Name

anti-PRPF8 antibody

Product Synonym Names

Mouse anti PRPF8; PRPF8 Antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

AB01/4B2

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).

Purity/Purification

Purified by affinity chromatograpy on Protein G from tissue culture supernatant

Preparation and Storage

Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch

Molecular Weight

252 kDa

OMIM

600059

3D Structure

ModBase 3D Structure for Q6P2Q9

Immunogen

E Coli-derived recombinant human PRPF8 (aa 1-467)

Lysate Composition

400ug HEK293 lysate lyophilized in RIPA buffer

Lysate Reconstitution

If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u

Applications Tested/Suitable for anti-PRPF8 antibody

Western Blot (WB)

Application Notes for anti-PRPF8 antibody

WB: 1:1000
Western Blotting: Detects a band of approximately 252 kDa in HEK293 cell lysates.

Other Notes

Small volumes of anti-PRPF8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRPF8. It may not necessarily be applicable to this product.

NCBI GI #

67460824

NCBI GeneID

10594

NCBI Accession #

Q6P2Q9.2

UniProt Primary Accession #

Q6P2Q9

UniProt Secondary Accession #

O14547; O75965

UniProt Related Accession #

Q6P2Q9

NCBI Official Full Name

Pre-mRNA-processing-splicing factor 8

NCBI Official Synonym Full Names

pre-mRNA processing factor 8

NCBI Official Symbol

PRPF8

NCBI Official Synonym Symbols

PRP8; RP13; HPRP8; PRPC8; SNRNP220

UniProt Protein Name

Pre-mRNA-processing-splicing factor 8

UniProt Synonym Protein Names

220 kDa U5 snRNP-specific protein; PRP8 homolog; Splicing factor Prp8; p220

Protein Family

Pre-mRNA-processing-splicing factor

UniProt Gene Name

PRPF8

UniProt Synonym Gene Names

PRPC8

NCBI Protein Information

pre-mRNA-processing-splicing factor 8

NCBI Summary for PRPF8

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]

UniProt Comments for PRPF8

Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

Research Articles on PRPF8

1. Multiple genes contributing to the retinal dystrophy genotypes within a family were discovered using retinal gene-targeted next-generation sequencing. Families with noted examples of phenotypic variation or apparent non-penetrant individuals may offer a clue to suspect complex inheritance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PRPF8 antibody

Reference Product	PubMed Publications
PRPF4 antibody	>2 publications with PRPF8 and PRPF4
PRPF19 antibody	>1 publications with PRPF8 and PRPF19
SLU7 antibody	>1 publications with PRPF8 and SLU7

Pathways associated with anti-PRPF8 antibody

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome, 35S U5-snRNP Pathway antibodies	Spliceosome, 35S U5-snRNP Pathway Diagram
Spliceosome, 35S U5-snRNP Pathway antibodies	Spliceosome, 35S U5-snRNP Pathway Diagram
Spliceosome, U4/U6.U5 Tri-snRNP Pathway antibodies	Spliceosome, U4/U6.U5 Tri-snRNP Pathway Diagram
Spliceosome, U4/U6.U5 Tri-snRNP Pathway antibodies	Spliceosome, U4/U6.U5 Tri-snRNP Pathway Diagram
MRNA Splicing Pathway antibodies	MRNA Splicing Pathway Diagram
MRNA Splicing - Major Pathway antibodies	MRNA Splicing - Major Pathway Diagram

Diseases associated with anti-PRPF8 antibody

Disease Name	Pubmed Publications
Retinitis Pigmentosa 13 Antibodies	>4 publications with PRPF8 and Retinitis Pigmentosa 13
Pancreatic Neoplasms Antibodies	>1 publications with PRPF8 and Pancreatic Neoplasms
Adenocarcinoma Antibodies	>1 publications with PRPF8 and Adenocarcinoma
Congenital Abnormalities Antibodies	>1 publications with PRPF8 and Congenital Abnormalities
Prostatic Neoplasms Antibodies	>1 publications with PRPF8 and Prostatic Neoplasms
Prostatic Diseases Antibodies	>1 publications with PRPF8 and Prostatic Diseases
Hyperplasia Antibodies	>1 publications with PRPF8 and Hyperplasia

Organs/Tissues associated with anti-PRPF8 antibody

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>18 publications with PRPF8 and Eye
Blood Antibodies	>4 publications with PRPF8 and Blood
Heart Antibodies	>3 publications with PRPF8 and Heart
Bone Antibodies	>2 publications with PRPF8 and Bone
Nerve Antibodies	>1 publications with PRPF8 and Nerve
Pancreas Antibodies	>1 publications with PRPF8 and Pancreas
Prostate Antibodies	>1 publications with PRPF8 and Prostate
Intestine Antibodies	>1 publications with PRPF8 and Intestine
Embryonic Tissue Antibodies	>1 publications with PRPF8 and Embryonic Tissue
Bone Marrow Antibodies	>1 publications with PRPF8 and Bone Marrow