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APPL1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225561

MBS225561 is an antibody targeting APPL1. Also known as Mouse anti APPL1. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
APPL1, Monoclonal Antibody
Full Product Name
Mouse anti APPL1
Product Gene Name
Product Synonym Names
APPL1 Antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
G03/2H2
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Purity/Purification
Purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation and Storage
Store undiluted at -20 degree C. Avoid repeated freeze/thaw cycles. Shelf Life: As supplied, 12 months from date of despatch
Molecular Weight
83 kDa
OMIM
604299
3D Structure
ModBase 3D Structure for Q9UKG1
Immunogen
E Coli-derived recombinant human APPL1 (aa 1-373)
Applications Tested/Suitable for anti-APPL1 antibody
Western Blot (WB)
Application Notes for anti-APPL1 antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 83 kDa in HEK293 cell lysates.

Western Blot (WB) of anti-APPL1 antibody

Western blot analysis of whole cell lysates probed with APPL1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 31 second exposure. Arrow points to APPL1 (molecular weight 83 kDa).
Mouse Anti-APPL1 Monoclonal Antibody – Cat# MBS225561

Other Notes

Small volumes of anti-APPL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for APPL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DCC-interacting protein 13-alpha
NCBI Official Synonym Full Names
adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
NCBI Official Symbol
APPL1
NCBI Official Synonym Symbols
APPL; MODY14; DIP13alpha
UniProt Protein Name
DCC-interacting protein 13-alpha
UniProt Synonym Protein Names
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
UniProt Gene Name
APPL1
UniProt Synonym Gene Names
; Dip13-alpha
NCBI Protein Information
DCC-interacting protein 13-alpha

NCBI Summary for APPL1

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]
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UniProt Comments for APPL1

Adapter protein that interacts with proteins involved in different cellular signaling pathways. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Involved in the regulation of the insulin receptor signaling pathway.
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Research Articles on APPL1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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