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Pulmonary Surfactant Associated Protein A (SPA) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS022557

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of SPA (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Pulmonary Surfactant Associated Protein A (SPA), ELISA Kit
Full Product Name
Mouse Pulmonary Surfactant Associated Protein A ELISA Kit
Product Gene Name
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Detection Range
6.25 pg/ml - 200 pg/ml
Sensitivity
1.0 pg/ml
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
26,702 Da
Chromosome Location
Chromosome: 14; NC_010456.4 (88698851..88702099, complement). Location: 14; 14
3D Structure
ModBase 3D Structure for P49874

Other Notes

Small volumes of SPA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPA purchase

MBS022557 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pulmonary Surfactant Associated Protein A (SPA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPA. The ELISA analytical biochemical technique of the MBS022557 kit is based on SPA antibody-SPA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Mutation of pulmonary surfactant protein A SPA are a leading cause of respiratory problems. Surfactant protein SPA is produced by human lung alveolar cells and is a component of a highly complex mix of lipids and lectins which reduce membrane surface tension and protect lung function during exhalation. To study this effect in depth, we offer a specfic sandwich SPA 1 ELISA kit from ELabScience. Manufactured to CLIA kits standards, you know you can trust this assay precision and results. Shipped at room temperature but store at 4oC. These ELISA kits are self contained, with polyclonal antibody plate, detection reagent, standard curve peptide, HRP conjugate secondary antibodies, wash solution, sample buffer and stop solution. Like all our top quality reagents, your samples of cell proteins will be assayed right for detection surfactant factors. Besides surfactant protein ELISA, our catalog ranges from stem cells, molecular biology reagents to purinergic receptor p2x.

Additional Details

NCBI/Uniprot data below describe general gene information for SPA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pulmonary surfactant-associated protein A
NCBI Official Symbol
SFTPA1
NCBI Official Synonym Symbols
PSP-A; SFTPA
UniProt Protein Name
Pulmonary surfactant-associated protein A
UniProt Gene Name
SFTPA1
UniProt Synonym Gene Names
SFTP1; SFTPA; PSAP; PSP-A; SP-A
UniProt Entry Name
SFTPA_PIG
NCBI Protein Information
pulmonary surfactant-associated protein A; PSAP; SP-A

UniProt Comments for SPA

Function: In presence of calcium ions, PSAP binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.

Subunit structure: Oligomeric complex of 6 set of homotrimers.

Subcellular location: Secreted › extracellular space › extracellular matrix. Secreted › extracellular space › surface film.

Miscellaneous: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Sequence similarities: Belongs to the SFTPA family.Contains 1 C-type lectin domain.Contains 1 collagen-like domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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