Hella Cells were treated with 10 mug BrdU for 1 hour (B) or left untreated (A). Cells were stained with rabbit anti-BrdU antibody at a dilution of 1/500. As a secondary antibody, sheep anti rabbit IgG DyLight 549 conjugated antibody was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.
BrdU-labeled led human lymphoma cells were stained with rabbit anti-BrdU antibody at a 1/50 dilution (A) and a 1/100 dilution (B). Sheep anti Rabbit IgG DyLight 488 conjugated antibody was used as the secondary detection reagent at a 1/50 dilution. READIDROP Propidium Iodide (1351101) was used to stain total DNA.
Jurkat cells were treated with 10 muM BrdU (A), CldU (B), IdU (C), EdU (D), Thymidine (E) or left untreated (F). Cells were stained with 1 mug/ml Rabbit anti BrdU antibody for 1 hour at room temperature. As a secondary antibody, Sheep anti Rabbit IgG DyLight488 conjugated antibody was used at a 1/100 dilution. Propidium Iodide was used to stain total DNA.
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