Figure A. Pacific Blue conjugated Mouse anti Human CD3 and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. Pacific Blue conjugated Mouse anti Human CD3 and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD55 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD55 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD55 . All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock (BUF070A).
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 and Alexa Fluor 488 conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 and Alexa Fluor 488 conjugated Mouse anti Human CD55 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 and FITC conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 and FITC conjugated Mouse anti Human CD55 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 700 conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 700 conjugated Mouse anti Human CD55 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse anti Human CD55 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 Cell Analyzer.
Published Customer Image:
Mouse Anti Human CD55 antibody, clone 67 used for the identification of CD55 expressing cells in rheumatoid arthritis synovial tissue by immunohistochemistry on cryostat sections.
Image caption:
Representative double staining of rheumatoid arthritis synovial tissue with antibodies against RasGRF1 and cell-specific markers. Synovial tissue sections were stained overnight with antibodies against Ras guanine nucleotide-releasing factor 1(RasGRF1), followed by antibodies against CD3, CD55, and CD68. After biotin tyramide enhancement, staining was developed with amino-ethylcarbazole (red, RasGRF1) and Fast blue (blue, cell-specific markers). Magnification x 100.
From: Abreu JR, de Launay D, Sanders ME, Grabiec AM, van de Sande MG, Tak PP, Reedquist KA.
The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue.
Arthritis Res Ther. 2009;11(4):R121.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Published Customer Image:
Mouse Anti CD55 antibofdy, clone 67 used for the demonstration of CD55 and MPGES1 in fibroblasts from sinovial lining by immunofluorescence.
Image caption:
MPGES1 expression in synovial lining fibroblasts before and 16 weeks after initiation of rituximab therapy.
Double immunofluorescence pictures show the presence of MPGES1 (red) expression in CD55 positive fibroblasts (green) in the rheumatoid tissue before rituximab initiation (A) and 16 weeks later (B). Original magnification 500x. Arrows point to double stained cells
From: Gheorghe KR, Thurlings RM, Westman M, Boumans MJ, Malmström V, Trollmo C, et al. (2011)
Prostaglandin E2 Synthesizing Enzymes in Rheumatoid Arthritis B Cells and the Effects of B Cell Depleting Therapy on Enzyme Expression.
PLoS ONE 6(1): e16378.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License. PLoS ONE 6(1): e16378.
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