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CD81 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225635

Mouse anti Human CD81 antibody, clone 1D6 recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD81, Monoclonal Antibody
Full Product Name
Mouse Anti Human CD81: RPE
Product Gene Name
Product Synonym Names
CD81 antibody (1D6); Mouse anti Human CD81
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1D6
Host
Mouse
Species Reactivity
Human, Chimpanzee, Sheep, Goat
Form/Format
Liquid; Phosphate Buffered Saline
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
OMIM
M33680 mRNA
3D Structure
ModBase 3D Structure for P60033
Immunogen
OCI-LY8 cells aggregated by 5A6 (another CD81 antibody)
Conjugation
RPE
Carrier Free
Yes
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
Preservative Stabilizers
0.09% Sodium Azide
Applications Tested/Suitable for anti-CD81 antibody
Flow Cytometry (FC/FACS)

Testing Data of anti-CD81 antibody

Figure A. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-CD81 Monoclonal Antibody – Cat# MBS225635

Testing Data of anti-CD81 antibody

Figure A. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 488 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 488 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Mouse Anti-CD81 Monoclonal Antibody, Testing Data – Cat# MBS225635

Testing Data of anti-CD81 antibody

Figure A. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Mouse Anti-CD81 Monoclonal Antibody, Testing Data – Cat# MBS225635

Testing Data of anti-CD81 antibody

Figure A. FITC conjugated Mouse anti Human CD19 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Mouse Anti-CD81 Monoclonal Antibody, Testing Data – Cat# MBS225635

Testing Data of anti-CD81 antibody

Figure A. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-CD81 Monoclonal Antibody, Testing Data – Cat# MBS225635

Other Notes

Small volumes of anti-CD81 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD81 purchase

MBS225635 is an antibody targeting CD81. Also known as Mouse Anti Human CD81: RPE. Reactive in Human, Chimpanzee, Sheep, Goat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD81. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
CD81 antigen
NCBI Official Synonym Full Names
CD81 molecule
NCBI Official Symbol
CD81
NCBI Official Synonym Symbols
S5.7; CVID6; TAPA1; TSPAN28
UniProt Protein Name
CD81 antigen
UniProt Synonym Protein Names
26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD_antigen: CD81
Protein Family
UniProt Gene Name
CD81
UniProt Synonym Gene Names
TAPA1; TSPAN28; Tspan-28
NCBI Protein Information
CD81 antigen

NCBI Summary for CD81

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
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UniProt Comments for CD81

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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