Figure A. Pacific Blue conjugated Rat anti Mouse CD11b and Alexa Fluor700 conjugated Rat IgG2b isotype control . Figure B. Pacific Blue conjugated Rat anti Mouse CD11b and Alexa Fluor700 conjugated Rat anti Mouse GR-1 . All experiments performed on red cell lysed mouse bone marrow gated on mononuclear cells in the presence of Mouse Seroblock (BUF041A). Data acquired on the ZE5 cell analyzer.

Figure A : Pacific Blue conjugated Rat anti Mouse CD11b and FITC conjugated Rat IgG2b isotype control . Figure B. Pacific Blue conjugated Rat anti Mouse CD11b and FITC conjugated Rat anti Mouse GR-1 . All experiments performed on red cell lysed mouse bone marrow gated on mononuclear cells in the presence of Mouse Seroblock (BUF041A). Data acquired on the ZE5 cell analyzer.

Figure A : RPE conjugated Rat anti Mouse CD11b and Alexa Fluor 647 conjugated Rat IgG2b isotype control . Figure B. RPE conjugated Rat anti Mouse CD11b and Alexa Fluor 647 conjugated Rat anti Mouse Gr-1 . All experiments performed on murine bone marrow in the presence of Murine SeroBlock (BUF041A).

Published customer image:
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the evaluation of Gr-1 expression on circulating monocytes from mouse blood by flow cytometry.
Image caption:
Depletion of the circulating monocyte and Gr1lo and F4/80hi populations following 1 week GW2580 treatment. A. Identification of total monocyte population in mouse blood using MAFIA-GFP. B Following one week of GW2580 treatment, no difference in total circulating monocytes was observed. FACS quantification of Gr1hi (M1) and Gr1lo (M2; C,D) and F4/80hi (E,F) in total monocytes following 1 week GW2580 treatment (n = 6 and n = 4 animals per group, respectively).
From: Leblond A-L, Klinkert K, Martin K, Turner EC, Kumar AH, Browne T, et al. (2015) Systemic and Cardiac Depletion of M2 Macrophage through CSF-1R Signaling Inhibition Alters Cardiac Function Post Myocardial Infarction.
PLoS ONE 10(9): e0137515.

Published customer image:
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the evaluation of Gr-1 expression on circulating monocytes from mouse blood by flow cytometry.
Image caption:
Effect of CD206+ M2 macrophage depletion on collagen deposition and cell infiltration within the infarct 2 weeks post MI in MAFIA mice. A. Representative Sirius Red staining on histological sections from MAFIA mice 2 weeks post MI. B. Quantification of collagen staining as a percentage of LV from MAFIA mice 2 weeks post MI (Scale bar: 1 mm, n = 6 animals per group, >=8 images per animal). C. Representative hematoxilin and eosin staining on 20x infarct or remote histological section from MAFIA mice 2 weeks post MI showing an increase in inflammatory infiltrates in animals treated with GW2580. D. Quantification of nuclei number per mm2 in MAFIA remote and infarct zone in MAFIA mice 2 weeks post MI. (n = 4-6 animals per group). E. Representative images of immunofluorescent staining of CD206+ M2 macrophages, Gr1+ M1 macrophages and Ly6G+ neutrophils within the infarct zone from MAFIA mice 2 weeks post MI. Quantification of Ly6G+ neutrophil, Gr1+ M1 macrophage and CD206+ M2 macrophage infiltration within the infarct zone from MAFIA mice 2 weeks post MI (n = 4 animals per group, 10 images/animal). * p<0.05, ** p<0.01.
From: Leblond A-L, Klinkert K, Martin K, Turner EC, Kumar AH, Browne T, et al. (2015) Systemic and Cardiac Depletion of M2 Macrophage through CSF-1R Signaling Inhibition Alters Cardiac Function Post Myocardial Infarction.
PLoS ONE 10(9): e0137515.

Published Customer Image:
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the identification of neutrophils by immunofluorescence.
Image caption:
S. typhimurium-Infected Macrophages Containing Phagocytosed Neutrophils and T Cells Confocal fluorescence microscopy of 50-mum-thick liver sections from 1-wk-infected Slc11a1 wild-type mice. (A-C) S.
Typhimurium (O-antigen, arrows) are red, macrophages (F4-80 and MOMA-2) are blue, DNA (DAPI) is gray, phalloidin is green, and neutrophils (Gr-1/Ly-6G/RB6-8C5) are pink (arrowheads). (A) Collapsed image from a 40-mum Z-stack. Scale bar is 20 mum. (B and C) Sections from (A) that are 4 mum apart. The video from which (A-C) were derived (Video S2) is available online. (D-G) T cells within multinucleate macrophages.
Macrophages (F4-80 and MOMA-2) are blue (D, G, and H), T cells (CD3zeta) are red (D, G, arrowheads), DAPI is gray (E, G), actin-bound phalloidin is green (F, G). (G) Is a composite of (D, E, and F). Scale bars are 16 mum. (H) An image from a different mouse stained and labeled as described for (D-G). Scale bar is 8 mum. A video showing a T cell inside of a macrophage is available online (Video S3).
From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.
PLoS Pathog 3(12): e193.