Figure A. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated Rat IgG2a isotype control . Figure B. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine peripheral blood in the presence of murine SeroBlock (BUF041A).

Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and Pacific Blue conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power

Rat anti Mouse CD31 antibody, clone ER-MP12 used for the detection of blood vessels in an experimental murine tumor model by immunohistofluorescence.
Image caption:
Percentage of pixels positive for NG2 in 4T1 (n = 5) and RM11 (n = 4 and n = 6) tumors (A,D) from WT and beta3-KO mice were calculated using immunofluorescent images. No statistical differences in 4T1 (p = 0.90) or RM11 (p = 0.23) were found. Mean +/- SD. Representative images of NG2-staining from both genotypes of 4T1 (B,C) (NG2 green, CD31 red) and RM11 (E,F) (NG2 red) tumors are shown. Scale bars indicate 50 mum.
From: Reigstad I, Sortland K, Skogstrand T, Reed RK, Stuhr L.
The Effect of Stromal Integrin beta3-Deficiency on Two Different Tumors in Mice.
Cancers (Basel). 2016 Jan 12;8(1). pii: E14.

Published Customer Image:
Mouse CD31 antibody, clone ER-MP12 used for the demonstration of vasculature in mouse brain by immunofluorescence.
Image caption:
Inhibition of 2-AG hydrolysis reduces LPS-induced BBB permeability. a, b Fibrinogen levels in b plasma and the a ratio of brain to plasma fibrinogen were assessed by ELISA. n?=?5/7 mice per group. c, d Fluorescent immunostaining in the striatum for fibrinogen (red) and vascular marker (CD31; green) demonstrated leakage of fibrinogen into the brain with vehicle treatment, whereas vascular integrity was preserved when (e, f) MAGL was inhibited. g Extravascular fibrinogen was semi-quantitated in fluorescently labeled sections of the striatum. Bar graphs were plotted with mean?+/-SEM and data analyzed using one-way analysis of variance (ANOVA) with Tukey post-hoc comparisons. n = 5/7 mice per group. Significance is shown as *p?<0.05, **p?<0.01. Scale bar = 20 mum.
From: Piro JR, Suidan GL, Quan J, Pi Y, O'Neill SM, Ilardi M, Pozdnyakov N, Lanz TA, Xi H, Bell RD, Samad TA.
Inhibition of 2-AG hydrolysis differentially regulates blood brain barrier permeability after injury.
J Neuroinflammation. 2018 May 14;15(1):142.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.