Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.
Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell monocytes. Data acquired on the ZE5 Cell Analyzer.
Figure A. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse IgG1 isotype control detected with FITC conjugated Goat anti Mouse IgG1 Figure B. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse anti Pig granulocytes detected with FITC conjugated Goat anti Mouse IgG1 . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.
Figure A. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse anti Pig granulocytes . All experiments performed on red cell lysed porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer
Published Customer Image:
Mouse Anti Porcine granulocytes antibody, clone 2B2 used to identify mature neutrophils in porcine blood by flow cytometery in conjunction with Mouse anti Porcine granulocytes antibody, clone 6D10 expressed on both immature and mature porcine neutrophils.
Image caption:
Gating strategy.
Granulocytes were further sub-divided into immature neutrophils (CD172a+ SSChigh 6D10+ 2B2-) and mature neutrophils (CD172a+ SSChigh 6D10+ 2B2+).
From: Nguyen DN, Jiang P, Frøkiær H, Heegaard PM, Thymann T, Sangild PT.
Delayed development of systemic immunity in preterm pigs as a model for preterm infants.
Sci Rep. 2016 Nov 10;6:36816.