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Granulocytes Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225668

Mouse anti Pig granulocytes antibody, clone 2B2 recognizes porcine basophils, neutrophils and eosinophils, acting as a reliable tool for their analysis and isolation, without contamination from other cells.Analysis of porcine bone marrow and blood shows that… More details

$500 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Granulocytes, Monoclonal Antibody
Full Product Name
Mouse Anti Pig Granulocytes
Product Synonym Names
Granulocytes antibody (2B2)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B2
Host
Mouse
Species Reactivity
Pig
Form/Format
Liquid; Phosphate Buffered Saline
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation and Storage
Store at 4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Immunogen
Porcine bone marrow hematopoietic cells (BMHC)
Fusion Partners
Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 mouse myeloma cell line.
Preservative Stabilizers
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Pack Size: 0.25 mg
0.09% Sodium Azide (NaN3)
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Applications Tested/Suitable for anti-Granulocytes antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-Granulocytes antibody
FC: 1/100-1/200

Testing Data of anti-Granulocytes antibody

Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-Granulocytes Monoclonal Antibody – Cat# MBS225668

Testing Data of anti-Granulocytes antibody

Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell monocytes. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-Granulocytes Monoclonal Antibody, Testing Data – Cat# MBS225668

Testing Data of anti-Granulocytes antibody

Figure A. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse IgG1 isotype control detected with FITC conjugated Goat anti Mouse IgG1 Figure B. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse anti Pig granulocytes detected with FITC conjugated Goat anti Mouse IgG1 . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-Granulocytes Monoclonal Antibody, Testing Data – Cat# MBS225668

Testing Data of anti-Granulocytes antibody

Figure A. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse anti Pig granulocytes . All experiments performed on red cell lysed porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer
Mouse Anti-Granulocytes Monoclonal Antibody, Testing Data – Cat# MBS225668

Testing Data of anti-Granulocytes antibody

Published Customer Image:
Mouse Anti Porcine granulocytes antibody, clone 2B2 used to identify mature neutrophils in porcine blood by flow cytometery in conjunction with Mouse anti Porcine granulocytes antibody, clone 6D10 expressed on both immature and mature porcine neutrophils.
Image caption:
Gating strategy.
Granulocytes were further sub-divided into immature neutrophils (CD172a+ SSChigh 6D10+ 2B2-) and mature neutrophils (CD172a+ SSChigh 6D10+ 2B2+).
From: Nguyen DN, Jiang P, Frøkiær H, Heegaard PM, Thymann T, Sangild PT.
Delayed development of systemic immunity in preterm pigs as a model for preterm infants.
Sci Rep. 2016 Nov 10;6:36816.
Mouse Anti-Granulocytes Monoclonal Antibody, Testing Data – Cat# MBS225668

Other Notes

Small volumes of anti-Granulocytes antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Granulocytes purchase

MBS225668 is an antibody targeting Granulocytes. Also known as Mouse Anti Pig Granulocytes. Reactive in Pig. Host: Mouse.
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