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IgE Antibody – Human Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225706

Description: Anti-IgE Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug omalizumab (Xolair) for research use. It can be used in bioanalytical assays and for studying biological pathways affected by the drug.Summary: Human Anti-IgE… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IgE, Monoclonal Antibody
Full Product Name
Human Anti IgE (Omalizumab Biosimilar)
Product Gene Name
anti-IgE antibody
Product Synonym Names
IgE antibody (huMaE11)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
huMaE11
Host
Human
Species Reactivity
Human
Form/Format
Liquid; Phosphate Buffered Saline
Purity/Purification
>98% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Immunogen
Human IgE protein
Preservative Stabilizers
0.02% Proclin 300
Applications Tested/Suitable for anti-IgE antibody
ELISA (EIA)

Testing Data of anti-IgE antibody

Omalizumab bridging ELISA for pharmacokinetic (PK) assay development
A microtiter plate was coated overnight with Human Anti-Omaliizumab Antibody (clone AbD20669, HCA236) at a concentration of 1 mug/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of omalizumab (therapeutic reference product) or Human Anti-IgE Antibody, MCA6105 (omalizumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Omalizumab Antibody (clone AbD20669_hIgG1, HCA235P) at a concentration of 2 mug/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human Anti-IgE Monoclonal Antibody – Cat# MBS225706

Testing Data of anti-IgE antibody

Purity assesment by SDS-PAGE
0.5 mug of Human Anti-IgE Antibody, MCA6105 (omalizumab biosimilar, research grade) and omalizumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Human Anti-IgE Monoclonal Antibody, Testing Data – Cat# MBS225706

Testing Data of anti-IgE antibody

Monodispersity assessment by HPLC-SEC
Size exclusion chromatography (SEC) of Human Anti-IgE Antibody, MCA6105 (omalizumab biosimilar, research grade) and omalizumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 mum; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 mul/min; inj. volume [mul]: 3; temp.: 25 degree C; detection: 280 nm; sample: mAb (1 mg/ml).
Human Anti-IgE Monoclonal Antibody, Testing Data – Cat# MBS225706

Other Notes

Small volumes of anti-IgE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IgE purchase

MBS225706 is an antibody targeting IgE. Also known as Human Anti IgE (Omalizumab Biosimilar). Reactive in Human. Host: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for IgE. It may not necessarily be applicable to this product.

NCBI GI #
22651682
NCBI Accession #
AAM45140.1
NCBI Official Full Name
immunoglobulin epsilon chain
Protein Family
IgE-binding protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IgE antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with IgE and FLNA

Pathways associated with anti-IgE antibody

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram

Organs/Tissues associated with anti-IgE antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with IgE and Bone
 Connective Tissue Antibodies  >4 publications with IgE and Connective Tissue
 Heart Antibodies  >3 publications with IgE and Heart
 Muscle Antibodies  >3 publications with IgE and Muscle
 Brain Antibodies  >3 publications with IgE and Brain
 Vascular Antibodies  >2 publications with IgE and Vascular
 Embryonic Tissue Antibodies  >2 publications with IgE and Embryonic Tissue
 Ear Antibodies  >1 publications with IgE and Ear
 Kidney Antibodies  >1 publications with IgE and Kidney
 Umbilical Cord Antibodies  >1 publications with IgE and Umbilical Cord
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