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IL6R Antibody – Human Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225707

Description: Anti-IL6R Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug tocilizumab (Actemra/RoActemra) for research use. It can be used in bioanalytical assays and for studying biological pathways affected by the drug.Summary: Human… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IL6R, Monoclonal Antibody
Full Product Name
Human Anti IL6R (Tocilizumab Biosimilar)
Product Gene Name
anti-IL6R antibody
Product Synonym Names
IL6R antibody (rhPM-1)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
rhPM-1
Host
Human
Species Reactivity
Human
Form/Format
Liquid; Phosphate Buffered Saline
Purity/Purification
>98% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
3D Structure
ModBase 3D Structure for A0N0L5
Immunogen
Mouse AUK 12-20 antibody CDRs grafted onto human framework
Preservative Stabilizers
0.02% Proclin 300
Applications Tested/Suitable for anti-IL6R antibody
ELISA (EIA)

Testing Data of anti-IL6R antibody

Tocilizumab bridging ELISA for pharmacokinetic (PK) assay development
A microtiter plate was coated overnight with Human Anti-Tocilizumab Antibody (clone AbD21362, HCA252) at a concentration of 1 mug/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of tocilizumab (therapeutic reference product) or Human Anti-IL6R Antibody, MCA6106 (tocilizumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Tocilizumab Antibody (clone AbD21338_hIgG1, HCA253P) at a concentration of 2 mug/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human Anti-IL6R Monoclonal Antibody – Cat# MBS225707

Testing Data of anti-IL6R antibody

Purity assessment by SDS-PAGE
0.5 mug of Human Anti-IL6R Antibody, MCA6106 (tocilizumab biosimilar, research grade) and tocilizumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Human Anti-IL6R Monoclonal Antibody, Testing Data – Cat# MBS225707

Testing Data of anti-IL6R antibody

Monodispersity assessment by HPLC-SEC
Size exclusion chromatography (SEC) of Human Anti-IL6R Antibody, MCA6106 (tocilizumab biosimilar, research grade) and tocilizumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 mum; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 mul/min; inj. volume [mul]: 3; temp.: 25 degree C; detection: 280 nm; sample: mAb (1 mg/ml).
Human Anti-IL6R Monoclonal Antibody, Testing Data – Cat# MBS225707

Other Notes

Small volumes of anti-IL6R antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL6R purchase

MBS225707 is an antibody targeting IL6R. Also known as Human Anti IL6R (Tocilizumab Biosimilar). Reactive in Human. Host: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for IL6R. It may not necessarily be applicable to this product.

UniProt Primary Accession #
A0N0L5
UniProt Protein Name
Interleukin 6 receptor
UniProt Synonym Protein Names
Interleukin 6 receptor
Protein Family
Interleukin-6 receptor
UniProt Gene Name
IL6R
UniProt Entry Name
A0N0L5_HUMAN

UniProt Comments for IL6R

IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; basolateral plasma membrane; apical plasma membrane; plasma membrane; extracellular region; interleukin-6 receptor complex

Molecular Function: protein binding; protein homodimerization activity; interleukin-6 receptor binding; interleukin-6 binding; enzyme binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of collagen biosynthetic process; neutrophil mediated immunity; positive regulation of interleukin-6 production; positive regulation of leukocyte chemotaxis; defense response to Gram-negative bacterium; endocrine pancreas development; positive regulation of chemokine production; positive regulation of tyrosine phosphorylation of Stat3 protein; activation of NF-kappaB transcription factor; monocyte chemotaxis; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; positive regulation of MAPKKK cascade; response to cytokine stimulus; positive regulation of cell proliferation; acute-phase response; negative regulation of interleukin-8 production; hepatic immune response

Disease: Interleukin 6, Serum Level Of, Quantitative Trait Locus; Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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