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TGF alpha Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225723

Recombinant human transforming growth factor (TGF) alphaGrowth factors such as TGF alpha and their respective receptors play a crucial role in regulating cell differentiation, proliferation and survival. TGF alpha stimulates cell proliferation and is… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TGF alpha, Active Protein
Full Product Name
Recombinant Human TGF alpha
Product Gene Name
TGF alpha active protein
Host
E Coli
Form/Format
Lyophilized
Purity/Purification
>=98% determined by silver staining of SDS-PAGE gel
Purified by ion exchange chromatography
Concentration
100 mug/ml after reconstitution (lot specific)
Preparation and Storage
Prior to reconstitution store at -20 degree C. Following reconstitution store at -20 degree C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
Should this product contain a precipitate we recommend microcentrifugation before use.
Molecular Weight
5.5 kDa
OMIM
190170
3D Structure
ModBase 3D Structure for P01135
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source
Human
Endotoxin Level
<1 EU/mug
Activity
Confirmed by performing an alamarBlue based cell proliferation assay using human breast cancer cells. The expected ED50 for this effect is 0.1-1 ng/ml.
Pre-Lyophilization Buffer
50 mM Tris-HCl, 0.15 M Sodium Chloride
Preservative Stabilizers
1.0% Trehalose
Reconstitution
Pack Size: 10 ug
Centrifuge vial prior to reconstitution. Reconstitute to 100 mug/ml by adding 100 mul ddH2O. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Pack Size: 100 ug
Centrifuge vial prior to reconstitution. Reconstitute to 100 mug/ml by adding 1 ml ddH2O. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Applications Tested/Suitable for TGF alpha active protein
Functional Assay

Testing Data of TGF alpha active protein

Silver staining of SDS-PAGE gel
Precision Plus Protein Dual Xtra Prestained Protein Standards were run in lane 1 and 1 mug Recombinant Human TGF Alpha was run in lane 2 on a 4-15% Mini-PROTEAN TGX Stain-Free Protein Gel. Silver staining was performed using the Silver Stain Plus Kit
Human TGF alpha Active Protein – Cat# MBS225723

Testing Data of TGF alpha active protein

The proliferative effect of recombinant human TGF alpha was demonstrated by performing a cell proliferation assay with human breast cancer cells using alamarBlue (BUF012A). The expected ED50 for this effect is 0.1-1 ng/ml
Human TGF alpha Active Protein, Testing Data – Cat# MBS225723

Other Notes

Small volumes of TGF alpha active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TGF alpha purchase

MBS225723 is an active protein targeting TGF alpha. Also known as Recombinant Human TGF alpha. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TGF alpha. It may not necessarily be applicable to this product.

NCBI GI #
153791414
NCBI GeneID
7039
NCBI Accession #
NP_001093161.1
UniProt Primary Accession #
P01135
UniProt Related Accession #
P01135
GenBank Nucleotide #
NM_001099691.2
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
UniProt Protein Name
Protransforming growth factor alpha
UniProt Synonym Protein Names
EGF-like TGF; ETGF; TGF type 1
Protein Family
Transforming growth factor
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
UniProt Entry Name
TGFA_HUMAN
NCBI Protein Information
protransforming growth factor alpha

NCBI Summary for TGF alpha

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
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UniProt Comments for TGF alpha

TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Membrane protein, integral; Cell cycle regulation

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: extracellular space; cell surface; integral to plasma membrane; perinuclear region of cytoplasm; basolateral plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus

Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to drug; cell proliferation; wound healing; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
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Research Articles on TGF alpha

1. The frequency of A allele in all the case groups and control groups was 50% and 2%, respectively, in the cleft lip cases was 50%, and in the cleft lip and palate cases was 50%. There was a statistically significant difference between genotype frequency of TGFalpha/HinfI polymorphisms in nsCL cases, nsCL+P cases, and nsCL +/- P cases compared with controls (p = 0.029, p = 0.024, and p = 0.0365, respectively).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TGF alpha active protein

 Reference Product  PubMed Publications
 EGFR active protein  >34 publications with TGF alpha and EGFR
 ERBB2 active protein  >7 publications with TGF alpha and ERBB2
 ERBB4 active protein  >3 publications with TGF alpha and ERBB4
 ADAM17 active protein  >1 publications with TGF alpha and ADAM17

Pathways associated with TGF alpha active protein

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 ErbB Receptor Signaling Network Pathway antibodies  ErbB Receptor Signaling Network Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 FOXM1 Transcription Factor Network Pathway antibodies  FOXM1 Transcription Factor Network Pathway Diagram
 Glioma Pathway antibodies  Glioma Pathway Diagram
 Glioma Pathway antibodies  Glioma Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram

Diseases associated with TGF alpha active protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >59 publications with TGF alpha and Neoplasms
 Genetic Predisposition to Disease Antibodies  >16 publications with TGF alpha and Genetic Predisposition to Disease
 Fatty Liver Antibodies  >16 publications with TGF alpha and Fatty Liver
 Liver Diseases Antibodies  >10 publications with TGF alpha and Liver Diseases
 Liver Neoplasms Antibodies  >10 publications with TGF alpha and Liver Neoplasms
 Head and Neck Neoplasms Antibodies  >9 publications with TGF alpha and Head and Neck Neoplasms
 Breast Neoplasms Antibodies  >9 publications with TGF alpha and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >7 publications with TGF alpha and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >7 publications with TGF alpha and Nervous System Diseases
 Carcinoma, Hepatocellular Antibodies  >7 publications with TGF alpha and Carcinoma, Hepatocellular

Organs/Tissues associated with TGF alpha active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with TGF alpha and Brain
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