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Alpha (CD8) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225809

Mouse anti Pig CD8 alpha antibody, clone 11/295/33 recognizes an epitope on the alpha chain of porcine CD8. Clone 11/295/33 detects a different epitope from that bound by clone 76-2-11.In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on… More details

$780 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD8 Alpha, Monoclonal Antibody
Full Product Name
Mouse Anti Pig CD8 Alpha: RPE
Product Synonym Names
CD8 Alpha antibody, 11/295/33
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
11/295/33
Host
Mouse
Species Reactivity
Pig
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - Lyophilized
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Immunogen
Porcine lymphocytes
Preservative Stabilizers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose
Reconstitution
Reconstitute with 1.0 ml distilled water
Applications Tested/Suitable for anti-CD8 Alpha antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD8 Alpha antibody
FC/FACS: Min. Dilution: Neat, Max. Dilution: 1/10
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Testing Data of anti-CD8 Alpha antibody

RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse IgG2a isotype control . Figure B. RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse anti Pig CD8 alpha . All experiments performed on porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-CD8 Monoclonal Antibody – Cat# MBS225809

Testing Data of anti-CD8 Alpha antibody

Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD8 alpha . All experiments performed on porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-CD8 Monoclonal Antibody, Testing Data – Cat# MBS225809

Testing Data of anti-CD8 Alpha antibody

FigureA.FITC conjugated Mouse anti Pig CD3 and Mouse IgG2a isotype control detected with Goat anti Mouse IgG2a:RPE . Figure B. FITC conjugated Mouse anti Pig CD3 and Mouse anti Pig CD8 alpha detected with Goat anti Mouse IgG2a:RPE . All experiments performed on red cell lysed porcine blood gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-CD8 Monoclonal Antibody, Testing Data – Cat# MBS225809

Testing Data of anti-CD8 Alpha antibody

Figure A. FITC conjugated Mouse anti Pig CD3 and RPE conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and RPE conjugated Mouse anti Pig CD8 alpha . All experiments performed on porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
Mouse Anti-CD8 Monoclonal Antibody, Testing Data – Cat# MBS225809

Other Notes

Small volumes of anti-CD8 Alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD8 Alpha purchase

MBS225809 is an antibody targeting CD8 Alpha. Also known as Mouse Anti Pig CD8 Alpha: RPE. Reactive in Pig. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD8 Alpha. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
CD8 alpha

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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