Ikaros (IKZF1) Antibody – Mouse Monoclonal

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]

Ikaros: a transcription factor of the ikaros C2H2-type zinc-finger protein family. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development. Deletions in Ikaros have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Seven alternatively spliced human isoforms have been described.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: centric heterochromatin; protein complex; cytoplasm; nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; protein heterodimerization activity; metal ion binding; transcription factor activity

Biological Process: Peyer's patch development; thymus development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of multicellular organism growth; chromatin modification; cell cycle; lymph node development; positive regulation of neutrophil differentiation; retina development in camera-type eye; natural killer cell differentiation; B cell differentiation; forebrain development; positive regulation of NK T cell differentiation; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; T cell differentiation